Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
- Winnie Contreras,
- ,
- Alexei Santiani
- Universidad Nacional Mayor de San Marcos,
Open access
Publication Information
Output type
Original language
SpanishArticle number
e17548Journal (Volume, Issue Number)
Revista de Investigaciones Veterinarias del Peru (Volume 31, Issue 1)Publication milestones
- Published - 29/03/2020
Publication status
ISSN
1682-3419Publication IDs
- Scopus: 85086161935
Abstract
The aim of this study was to determine the effect of the type (dimethylacetamide [DMA] and dimethylformamide [DMF]) and concentrations (1, 3.5, 7%) of cryoprotective agents on three sperm parameters during the cryopreservation process of epididymal alpaca sperm. A 2x3 factorial design consisting of the two cryoprotectants and the three concentrations was used. The parameters of sperm viability (SYBR-14/PI) and mitochondrial membrane potential (MitoTracker Deep Red FM) of sperm of 40 epididymis were evaluated by flow cytometry and motility by microscopy after thawing. A higher percentage of motility after thawing was found with AMD (14%) than with DMF (10%) (p=0.001). In addition, there was a significant effect of the cryoprotectant concentration in all the parameters evaluated (p<0.001), being better at concentrations of 1 and 3.5% than with 7%. No significant effect of the cryoprotectant-concentration interaction was found.