Phagocytosis stimulation in Oncorhynchus mykiss juveniles immunized with outer membrane fractions from a Flavobacterium psychrophilum native strain

Original title: Estimulación de la fagocitosis en juveniles de Oncorhynchus mykiss inmunizados con fracciones de membrana externa de una cepa nativa de Flavobacterium psychrophilum

E. Colona Vallejos(corresponding author)
,
A. Mayanga Herrera
,
C. De Amat Herbozo
,
L. Alzamora Gonzales
,
J. Elías Paredes
,
R. Echevarría Del Valle

*Corresponding author for this work

Universidad Nacional Mayor de San Marcos
,
Medicina humana

Research Output:

Contribution to journal

Article

Peer-review

Publication Information

Output type

Research Output:

Contribution to journal

Article

Peer-review

Original language

Spanish

Article number

011305

Journal (Volume, Issue Number)

Revista Electronica de Veterinaria (Volume 14, Issue 1)

Publication milestones

Published - 01/2013

Publication status

Published - 01/2013

Publication IDs

Scopus: 84874638219

Abstract

The aim of this study was to evaluate phagocytosis stimulation in Oncorhynchus mykiss (rainbow trout) juveniles immunized with outer membrane fraction (OMF) from Flavobacterium psychrophilum USM-3 native strain and challenged with Flavobacterium psychrophilum 1947^T standard strain. Fifteen specimens were immunized intraperitoneally with 10 μg of OMF and fifteen specimens with 0.1ml of bidistilled water. Afterwards, both groups were challenged with Flavobacterium psychrophilum 1947^T standard strain. After 21 days from first immunization, phagocytic activity and index were determined. Mean phagocytic activity in immunized fish was 22.4% mean while in control group it was 0.5% (p<0.05). Phagocytic index percentage in immunized fish and control group was 32.5% and 0.5% (p<0.05) respectively. It was concluded that outer membrane fraction from Flavobacterium psychrophilum USM-3 native strain stimulates phagocytosis in Oncorhynchus mykiss juveniles infected with F. psychrophilum 1947^T.