Detection of dihydrofolate reductase genes by PCR and RFLP
- Margarita M. Navia,
- ,
- Javier Sanchez-Cespedes,
- Jordi Vila
- Departament de Microbiologia,
Research Output:
Contribution to journal
Article
Peer-reviewPublication Information
Output type
Research Output:
Contribution to journal
Article
Peer-reviewOriginal language
EnglishPages from-to (Number of pages)
Pages 295-298 (4 pages)Journal (Volume, Issue Number)
Diagnostic Microbiology and Infectious Disease (Volume 46, Issue 4)Publication milestones
- Published - 01/08/2003
Publication status
Published - 01/08/2003
ISSN
0732-8893Publication IDs
- Scopus: 0041424806
- PubMed: 12944023
Abstract
The presence of plasmid-encoded trimethoprim resistant dfr genes is the most common mechanism responsible for the acquisition of trimethoprim resistance. The usual method to detect the presence of these genes is hybridization with specific probes. We describe an alternative, faster and easier method, based on PCR amplification and RFLP analysis, to discriminate up to sixteen different dfr genes.
Funding Details
We thank Dr. S.G.B. Amyes for kindly providing some of the strains. This work was partially supported by grant FIS00/0997 from the Fondo de Investigaciones Sanitarias (Spain). JSC has a grant associated to project PETRI no. PTR1995-0430-OP
FundersFunding numbers
Fondo de Investigaciones Sanitarias
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SDG 3 Good Health and Well