Abamectin and emamectin in grapes of Vitis vinifera L. from a district of the Valley of Ica-Peru

Context: In 14 districts of the valley of Ica-Peru, Vitis vinifera L. plants are cultivated that produce grapes for consumption as table grapes and raisins (dried grapes); at the same time, for the production of wines and Piscos. Aims: To determine the levels of abamectin and emamectin in grapes of Vitis vinifera L from a district of the Valley of Ica-Peru. Methods: 30 lots (30 kg) of Moscatel grape variety V. vinifera L. were collected from six countryside (artisanal and organic cultivation) of the San Juan Bautista district. The extraction of abamectin (ABM) and emamectin benzoate (EMB) from the grapes was carried out with acetonitrile; it was quantified by means of Liquid Chromatography coupled to Mass Spectrometry (HPLC-MS). The maximum permissible limit values (MRL) were established at 0.010 ppm for both insecticides. Results: The determined levels of abamectin and emamectin in grapes were 0.0012-0.015 ppm and 0.0013-0.013 ppm, respectively. Values higher than the maximum permissible limits of abamectin were found in batches A2 (0.0102 ppm), C1 (0.015 ppm), C5 (0.0113 ppm), and F2 (0.012 ppm); emamectin benzoate in lots B1 (0.0113 ppm), B4 (0.013 ppm) and C4 (0.012 ppm). Using the Shapiro-Wilk, Anderson Darling and Student's t tests, it was found that the global means of the values of the two insecticides in grapes are lower than the MRL. According to the global analysis of variance, the means of the concentrations of both insecticides were not different between the six sampling zones (countryside). Conclusions: The insecticides abamectin and emamectin are below the maximum permissible limit values (0.010 ppm) in Moscatel grapes of Vitis vinifera L., so the residual effect would not have implications for human health.