@article{d8cfaafa802e4e2da13751967434f71b,
title = "Occurrence of a sibling species complex within neotropical lymnaeids, snail intermediate hosts of fascioliasis",
abstract = "The delimitation of cryptic species within the genus Lymnaea, which are the main vectors of fascioliasis, remains a topic of controversy. An analysis of genetic variability based on 12 enzyme loci revealed different fixed alleles at 9 loci between two sympatric samples of Lymnaea viatrix at the type locality in Lima, Peru. The absence of heterozygotes within this locality indicates the presence of isolated populations or cryptic species within L. viatrix. Significant genetic differences were also found between these two L. viatrix samples from Lima and other populations of L. viatrix in South America and in addition to species such as L. truncatula, L. cubensis and L. columella. Moreover, the lack of variability within each Lymnaea samples studied indicates the existence of a high selfing rate in each species.",
keywords = "Lymnaea, Mollusc, Multilocus enzyme electrophoresis, Phylogeography, Population genetics",
author = "P. Durand and Pointier, {J. P.} and K. Escoubeyrou and Arenas, {J. A.} and M. Yong and M. Amarista and Bargues, {M. D.} and S. Mas-Coma and F. Renaud",
note = "Funding Information: We thank Hakima Yahia for technical assistance and Philip Agnew for comments and English corrections. This work was partly supported by funding from the Centre National de la Recherche Scientifique (C.N.R.S) and the Programme of Scientific Cooperation with Latin America, Instituto de Cooperaci{\'o}n Iberoamericana, Agencia Espa{\~n}ola de Cooperaci{\'o}n Internacional (I.C.I.-A.E.C.I.), Madrid, Spain, DGICYT Proyect No. PM97-0099 of the Spanish Ministry of Education and Culture, Madrid, and Project No. 3006 of the Direcci{\'o}n General de Cooperaci{\'o}n para el Desarrollo, Presidencia de Gobierno de la Generalitat Valenciana, Valencia, Spain. Samples of lymnaeid snails from Portugal and Argentina were kindly supplied by Dr M.A. Concei{\c c}{\~a}o, Instituo de Sa{\'u}de, Departamento de Parasitologia, Oporto, and Dr N. Cazzaniga, Departamento de Biologia, Bioquimica y Farmacia, Universidad Nacional del Sur, Bahia Blanca.",
year = "2002",
month = sep,
doi = "10.1016/S0001-706X(02)00128-6",
language = "Ingl{\'e}s",
volume = "83",
pages = "233--240",
journal = "Acta Tropica",
issn = "0001-706X",
publisher = "Elsevier",
number = "3",
}