TY - JOUR
T1 - In vitro selected fluoroquinolone-resistant mutants of Citrobacter freundii
T2 - Analysis of the quinolone resistance acquisition
AU - Del Mar Tavío, María
AU - Vila, Jordi
AU - Ruiz, Joaquím
AU - Amicosante, Gianfranco
AU - Franceschini, Nicola
AU - Martín-Sánchez, Antonio M.
AU - Jiménez De Anta, María Teresa
PY - 2000
Y1 - 2000
N2 - Ten quinolone-resistant mutants of Citrobacter freundii, which were selected in vitro with fluoroquinolones from two clinical isolates, were studied. The parent isolates were susceptible to quinolones in spite of showing a single substitution in the GyrB (His-417→Leu). No change was observed in the outer membrane proteins or in the lipopolysaccharide in any of the ten mutants studied with respect to their parent isolates. The development of quinolone resistance in selected mutants was associated with the appearance of a substitution in the GyrA (Thr-83→Ile) in nine of the ten mutants plus enhanced active efflux in all of them.
AB - Ten quinolone-resistant mutants of Citrobacter freundii, which were selected in vitro with fluoroquinolones from two clinical isolates, were studied. The parent isolates were susceptible to quinolones in spite of showing a single substitution in the GyrB (His-417→Leu). No change was observed in the outer membrane proteins or in the lipopolysaccharide in any of the ten mutants studied with respect to their parent isolates. The development of quinolone resistance in selected mutants was associated with the appearance of a substitution in the GyrA (Thr-83→Ile) in nine of the ten mutants plus enhanced active efflux in all of them.
UR - http://www.scopus.com/inward/record.url?scp=0034114295&partnerID=8YFLogxK
U2 - 10.1093/jac/45.4.521
DO - 10.1093/jac/45.4.521
M3 - Artículo
C2 - 10747831
AN - SCOPUS:0034114295
SN - 0305-7453
VL - 45
SP - 521
EP - 524
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
IS - 4
ER -
