Immunogenic peptides from Pap31 and SCS-α of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease

Cláudia Gomes, Maria J. Pons, Juana Del Valle-Mendoza, Mayumi Matsuoka, Joaquim Ruiz

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

3 Citas (Scopus)

Resumen

Bartonella bacilliformis is the causal agent of Carrion’s disease, an overlooked illness endemic in the Andean Mountains with Peru being the most affected country. The diagnostic of this illness is a challenge due to the limited resources and the common symptomatology with other infectious diseases. The goal of this study was to identify immunogenic peptides from Pap31 and succinyl-CoA synthetase α (SCS-α) of B. bacilliformis that might be suitable for developing a serologic tool. The immunodominant character of Pap31 and SCS-α was determined byWestern blotting and in-silico analysis. Subsequently, 35 peptides were selected for epitope mapping and their immunoreactivity was tested by enzyme-linked immunosorbent assay (ELISA). A total of 30 sera were tested including pre-exposed people with high IgM levels for Pap31/SCS-α (23 sera), patients (2 sera) as well as 5 sera with no reactivity to Pap31/SCS-α. The results indicate that Pap31-8 (187QAIGSAILKGTKDTGT202) and SCS-α-12 (59IFASVAEGKEKTGANA74) are the most immunogenic peptides, with Pap31-8 showing potential to discriminate between B. bacilliformis and the remaining Bartonella spp., and SCS-α-12 differentiating Bartonella spp. from other microorganisms.

Idioma originalInglés
Número de artículo917
PublicaciónPathogens
Volumen10
N.º8
DOI
EstadoPublicada - ago. 2021

Huella

Profundice en los temas de investigación de 'Immunogenic peptides from Pap31 and SCS-α of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease'. En conjunto forman una huella única.

Citar esto