Identificación de micobacterias en medio sólido mediante microscopía de fase invertida y tinción Ziehl-Neelsen

Jesús Rojas Jaimes, Jorge Giraldo-Chavez, Yudit Huyhua-Flores, Tatiana Caceres-Nakiche

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

With the aim of identifying morphological differences in colonies of different species of mycobacteria by means of inverted phase microscopy, nine species of high-prevalence pathogenic mycobacteria were seeded in 7H11 solid medium and were observed for up to 21 days. Additionally, Ziehl-Neelsen (ZN) stains were carried out for each one of them. No morphological variations were identified among the species grown on solid medium; however, it was identified that all are able to form cords in solid medium in an early stage of growth, being corroborated by the Ziehl-Neelsen stain in Mycobacterium tuberculosis. so techniques based on detecting M. tuberculosis depending on the identification of the cords should be taken with caution so as not to generate a bad diagnosis, so microbiological characteristics could also be established as a minimum time to detect growth, temperature of growth and bacillary characteristics based on the Ziehl-Neelsen stain for each species studied.

Título traducido de la contribuciónIdentification in solid culture by inverted phase microscopy and Ziehl-Neelsen stain
Idioma originalEspañol
Páginas (desde-hasta)279-284
Número de páginas6
PublicaciónRevista Peruana de Medicina Experimental y Salud Publica
Volumen35
N.º2
DOI
EstadoPublicada - 1 abr. 2018

Palabras clave

  • Culture media (source: MeSH NLM)
  • Mycobacterias
  • Mycobacterium tuberculosis
  • Tuberculosis

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