TY - JOUR
T1 - Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila
AU - Claudiano, Gustavo S.
AU - Yunis-Aguinaga, Jefferson
AU - Marinho-Neto, Fausto A.
AU - Miranda, Renata L.
AU - Martins, Isabela M.
AU - Otani, Fabrizia S.
AU - Mundim, Antonio V.
AU - Marzocchi-Machado, Cleni M.
AU - Moraes, Julieta R.E.
AU - de Moraes, Flávio Ruas
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/5
Y1 - 2019/5
N2 - The pathogenesis of sepsis involves complex systems and multiple interrelationships between the host and pathogen producing high mortality rates in various animal species. In this study, hematological disturbances, innate immunity and survival during the septic process in Piaractus mesopotamicus inoculated with Aeromonas hydrophila were studied. For this aim, fish blood samples were taken from control and infected groups 1, 3, 6, and 9 h post-inoculation (HPI). Leukogram showed reduction in the number of leukocytes and thrombocytes, followed by cessation of leukocyte chemotaxis 6 HPI and severe morphological changes in leukocytes and erythrocytes. At 3 HPI production of reactive oxygen species increased and at 6 HPI decreased. There was no change in serum lysozyme concentration and lytic activity of the complement system, despite the progressive increase in serum lytic activity and bacterial agglutination. Finally, the changes in clinical signs due to aeromonosis and increasing septicemia resulted in a reduction in survival to 57.14% after 36 HPI. It was possible concluded that these hematological and immune are crucial event in the worsening of sepsis in P. mesopotamicus, and these findings are utility for diagnosing and understanding the pathophysiology sepsis in pacu induced by A. hydrophila.
AB - The pathogenesis of sepsis involves complex systems and multiple interrelationships between the host and pathogen producing high mortality rates in various animal species. In this study, hematological disturbances, innate immunity and survival during the septic process in Piaractus mesopotamicus inoculated with Aeromonas hydrophila were studied. For this aim, fish blood samples were taken from control and infected groups 1, 3, 6, and 9 h post-inoculation (HPI). Leukogram showed reduction in the number of leukocytes and thrombocytes, followed by cessation of leukocyte chemotaxis 6 HPI and severe morphological changes in leukocytes and erythrocytes. At 3 HPI production of reactive oxygen species increased and at 6 HPI decreased. There was no change in serum lysozyme concentration and lytic activity of the complement system, despite the progressive increase in serum lytic activity and bacterial agglutination. Finally, the changes in clinical signs due to aeromonosis and increasing septicemia resulted in a reduction in survival to 57.14% after 36 HPI. It was possible concluded that these hematological and immune are crucial event in the worsening of sepsis in P. mesopotamicus, and these findings are utility for diagnosing and understanding the pathophysiology sepsis in pacu induced by A. hydrophila.
KW - Bacteria
KW - Blood
KW - Innate immunity
KW - Leukocytes
KW - Pacu
UR - http://www.scopus.com/inward/record.url?scp=85062516579&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2019.01.044
DO - 10.1016/j.fsi.2019.01.044
M3 - Artículo
C2 - 30716521
AN - SCOPUS:85062516579
SN - 1050-4648
VL - 88
SP - 259
EP - 265
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
ER -