Células madre adiposas humanas disminuyen el daño de la fibrosis hepática con baja persistencia de células trasplantadas en ratas

Nathaly Enciso Benavides, Carlos Cisneros Huamaní, Nancy Rojas Morán, Ernesto Nava Carrión, John Pando Mayta, Fredy Fabián Domínguez, José Amiel Pérez, Javier Enciso Gutiérrez

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

In chronic liver disease, orthotopic transplantation is the only current therapeutic alternative but it is limited due to lack of donors. Trials with adult stem cells in acute liver damage show promising results. The aim of this work was to evaluate the effectiveness of human adipose stem cell (h-ASC) infusion in rats with chronic liver damage. Rats with thioacetamide-induced liver fibrosis were grouped into: group I control that did not receive thioacetamide and h-ASC, group II received thioacetamide and saline i.v., group III received thioacetamide and h-ASC 1 x 106/ kg i.v. via tail vein. Histological liver regeneration was evaluated by METAVIR index, while Macrophagocytus stellatus (Kupffer cells), stellate cells a-SMA+ and collagen I+ cells by immunohistochemistry; functional damage was evaluated by blood levels of the analytes Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Urea and Blood Urea Nitrogen (BUN) and hemogram. The results show attenuation of structural liver damage evidenced by decreased nodules, degree of histologic injury on Metavir score, and decreased Macrophagocytus stellatus, a-SMA+ cells and type I+ collagen cells; functionally there is moderate reduction of AST, ALT, urea, BUN and moderate decrease of white cells but favorable effect on mean corpuscular volume and mean corpuscular hemoglobin. Eight weeks after infusion there is a small population of h-ASC in the liver. In conclusion, intravenous infusion of h-ASC in rats reduces functional and structural damage of hepatic fibrosis with low persistence of h-ASC in the liver parenchyma. To our knowledge this is the first work that evaluates the effect of h-SC in the model of chronic murine liver damage and the persistence of transplanted cells.

Título traducido de la contribuciónHuman adipose stem cells decrease liver fibrosis damage with low persistence of transplanted cells in rats
Idioma originalEspañol
Páginas (desde-hasta)1496-1507
Número de páginas12
PublicaciónInternational Journal of Morphology
Volumen38
N.º5
DOI
EstadoPublicada - oct. 2020

Palabras clave

  • Adipose stem cells
  • Cell therapy
  • Hepatic fibrosis, xenotransplantation

Huella

Profundice en los temas de investigación de 'Células madre adiposas humanas disminuyen el daño de la fibrosis hepática con baja persistencia de células trasplantadas en ratas'. En conjunto forman una huella única.

Citar esto