TY - JOUR
T1 - Antiviral activity of the sea cucumber tegument extract (Pattalus mollis) on human rotavirus A (RVA)
AU - Garcia-Candela, Enrique
AU - Pariona-Velarde, Carlos
AU - Mondragón-Martínez, Aarón
AU - Chumpitaz-Cerrate, Victor
N1 - Publisher Copyright:
© 2019 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - The antiviral effect against RVA in cell culture was evaluated by using an aqueous extract of Patallus mollis sea cucumber, applying the titration methodology. This technique is used to measures the ability of the extract dilutions to inhibit the cytopathic effect (CPE) of the virus, expressed as percentage of inhibition (IP). The mean extract cytotoxic concentration (CC50) used in the antiviral assay was 27,042.10 μg/mL and the PI of the antiviral activity extract was greater than 99.9% for each concentration. To determine the viral action mode, the cells were previously treated with the extracts in different stages during the viral infection cycle. The result analysis suggests that the extract inhibits 99% of the virus during the absorption and viral inactivation phase. These results show the P. mollis extract has a remarkable antiviral effect against the RVA in cell culture. So that, it is crucial to investigate its action mechanisms.
AB - The antiviral effect against RVA in cell culture was evaluated by using an aqueous extract of Patallus mollis sea cucumber, applying the titration methodology. This technique is used to measures the ability of the extract dilutions to inhibit the cytopathic effect (CPE) of the virus, expressed as percentage of inhibition (IP). The mean extract cytotoxic concentration (CC50) used in the antiviral assay was 27,042.10 μg/mL and the PI of the antiviral activity extract was greater than 99.9% for each concentration. To determine the viral action mode, the cells were previously treated with the extracts in different stages during the viral infection cycle. The result analysis suggests that the extract inhibits 99% of the virus during the absorption and viral inactivation phase. These results show the P. mollis extract has a remarkable antiviral effect against the RVA in cell culture. So that, it is crucial to investigate its action mechanisms.
KW - Antiviral activity
KW - Patallus mollis
KW - human rotavirus type A (RVA)
KW - marine organism
KW - sea cucumber
UR - http://www.scopus.com/inward/record.url?scp=85072014763&partnerID=8YFLogxK
U2 - 10.1080/14786419.2019.1611807
DO - 10.1080/14786419.2019.1611807
M3 - Artículo
C2 - 31496276
AN - SCOPUS:85072014763
SN - 1478-6419
VL - 35
SP - 1014
EP - 1018
JO - Natural Product Research
JF - Natural Product Research
IS - 6
ER -