Validación de la técnica de PCR para la detección de Escherichia coli enteroagregativa causante de diarrea del viajero

Joaquim Ruiz-Blázquez; Martha Vargas; James P. Nataro; Jordi Vila; Joaquim Gascón I Brustenga

doi:10.1157/13078826

Validación de la técnica de PCR para la detección de Escherichia coli enteroagregativa causante de diarrea del viajero

Translated title of the contribution: Validation of a PCR technique for the detection of enteroagreggative Escherichia coli causing traveler's diarrhea

Joaquim Ruiz-Blázquez, Martha Vargas, James P. Nataro, Jordi Vila, Joaquim Gascón I Brustenga

Medicina humana

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

INTRODUCTION. Detection of enteroaggregative strains of Escherichia coli (EAEC) by the gold standard technique (cellular adherence) is difficult to implement in routine clinical practice. METHODS. The gold standard was compared with a PCR method for this purpose. RESULTS. With respect to the cellular adherence technique, PCR performance was as follows: sensitivity 0.93, specificity 1, positive predictive value 1, and negative predictive value 0.91. CONCLUSION. The PCR method studied is a viable alternative for routine detection of EAEC strains.

Translated title of the contribution	Validation of a PCR technique for the detection of enteroagreggative Escherichia coli causing traveler's diarrhea
Original language	Spanish
Pages (from-to)	479-481
Number of pages	3
Journal	Enfermedades Infecciosas y Microbiologia Clinica
Volume	23
Issue number	8
DOIs	https://doi.org/10.1157/13078826
State	Published - Oct 2005

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title = "Validaci{\'o}n de la t{\'e}cnica de PCR para la detecci{\'o}n de Escherichia coli enteroagregativa causante de diarrea del viajero",

abstract = "INTRODUCTION. Detection of enteroaggregative strains of Escherichia coli (EAEC) by the gold standard technique (cellular adherence) is difficult to implement in routine clinical practice. METHODS. The gold standard was compared with a PCR method for this purpose. RESULTS. With respect to the cellular adherence technique, PCR performance was as follows: sensitivity 0.93, specificity 1, positive predictive value 1, and negative predictive value 0.91. CONCLUSION. The PCR method studied is a viable alternative for routine detection of EAEC strains.",

keywords = "Diarrhea, EAEC, HEp-2",

author = "Joaquim Ruiz-Bl{\'a}zquez and Martha Vargas and Nataro, {James P.} and Jordi Vila and {Gasc{\'o}n I Brustenga}, Joaquim",

year = "2005",

month = oct,

doi = "10.1157/13078826",

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T1 - Validación de la técnica de PCR para la detección de Escherichia coli enteroagregativa causante de diarrea del viajero

AU - Ruiz-Blázquez, Joaquim

AU - Vargas, Martha

AU - Nataro, James P.

AU - Vila, Jordi

AU - Gascón I Brustenga, Joaquim

PY - 2005/10

Y1 - 2005/10

N2 - INTRODUCTION. Detection of enteroaggregative strains of Escherichia coli (EAEC) by the gold standard technique (cellular adherence) is difficult to implement in routine clinical practice. METHODS. The gold standard was compared with a PCR method for this purpose. RESULTS. With respect to the cellular adherence technique, PCR performance was as follows: sensitivity 0.93, specificity 1, positive predictive value 1, and negative predictive value 0.91. CONCLUSION. The PCR method studied is a viable alternative for routine detection of EAEC strains.

AB - INTRODUCTION. Detection of enteroaggregative strains of Escherichia coli (EAEC) by the gold standard technique (cellular adherence) is difficult to implement in routine clinical practice. METHODS. The gold standard was compared with a PCR method for this purpose. RESULTS. With respect to the cellular adherence technique, PCR performance was as follows: sensitivity 0.93, specificity 1, positive predictive value 1, and negative predictive value 0.91. CONCLUSION. The PCR method studied is a viable alternative for routine detection of EAEC strains.

KW - Diarrhea

KW - EAEC

KW - HEp-2

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DO - 10.1157/13078826

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JO - Enfermedades Infecciosas y Microbiologia Clinica

JF - Enfermedades Infecciosas y Microbiologia Clinica

IS - 8

ER -

Validación de la técnica de PCR para la detección de Escherichia coli enteroagregativa causante de diarrea del viajero

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