TY - JOUR
T1 - Traditional marketed meats as a reservoir of multidrug-resistant Escherichia coli
AU - Guibert, Fernando
AU - Espinoza, Kathya
AU - Taboada-Blanco, Clara
AU - Alonso, Carla A.
AU - Oporto, Rosario
AU - Castillo, Angie K.
AU - Rojo-Bezares, Beatriz
AU - López, María
AU - Sáenz, Yolanda
AU - Pons, Maria J.
AU - Ruiz, Joaquim
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
PY - 2023
Y1 - 2023
N2 - This study aimed to analyze Escherichia coli from marketed meat samples in Peru. Sixty-six E. coli isolates were recovered from 21 meat samples (14 chicken, 7 beef), and antimicrobial resistance levels and the presence of mechanisms of antibiotic resistance, as well as clonal relationships and phylogeny of colistin-resistant isolates, were established. High levels of antimicrobial resistance were detected, with 93.9% of isolates being multi-drug resistant (MDR) and 76.2% of samples possessing colistin-resistant E. coli; of these, 6 samples from 6 chicken samples presenting mcr-1-producer E. coli. Colistin-resistant isolates were classified into 22 clonal groups, while phylogroup A (15 isolates) was the most common. Extended-spectrum β-lactamase- and pAmpC-producing E. coli were found in 18 and 8 samples respectively, with bla CTX-M-55 (28 isolates; 16 samples) and bla CIT (8 isolates; 7 samples) being the most common of each type. Additionally, bla CTX-M-15, bla CTX-M-65, bla SHV-27, bla OXA-5/10-like, bla DHA, bla EBC and narrow-spectrum bla TEM were detected. In addition, 5 bla CTX-M remained unidentified, and no sought ESBL-encoding gene was detected in other 6 ESBL-producer isolates. The tetA, tetE and tetX genes were found in tigecycline-resistant isolates. This study highlights the presence of MDR E. coli in Peruvian food-chain. The high relevance of CTX-M-55, the dissemination through the food-chain of pAmpC, as well as the high frequency of unrelated colistin-resistant isolates is reported.
AB - This study aimed to analyze Escherichia coli from marketed meat samples in Peru. Sixty-six E. coli isolates were recovered from 21 meat samples (14 chicken, 7 beef), and antimicrobial resistance levels and the presence of mechanisms of antibiotic resistance, as well as clonal relationships and phylogeny of colistin-resistant isolates, were established. High levels of antimicrobial resistance were detected, with 93.9% of isolates being multi-drug resistant (MDR) and 76.2% of samples possessing colistin-resistant E. coli; of these, 6 samples from 6 chicken samples presenting mcr-1-producer E. coli. Colistin-resistant isolates were classified into 22 clonal groups, while phylogroup A (15 isolates) was the most common. Extended-spectrum β-lactamase- and pAmpC-producing E. coli were found in 18 and 8 samples respectively, with bla CTX-M-55 (28 isolates; 16 samples) and bla CIT (8 isolates; 7 samples) being the most common of each type. Additionally, bla CTX-M-15, bla CTX-M-65, bla SHV-27, bla OXA-5/10-like, bla DHA, bla EBC and narrow-spectrum bla TEM were detected. In addition, 5 bla CTX-M remained unidentified, and no sought ESBL-encoding gene was detected in other 6 ESBL-producer isolates. The tetA, tetE and tetX genes were found in tigecycline-resistant isolates. This study highlights the presence of MDR E. coli in Peruvian food-chain. The high relevance of CTX-M-55, the dissemination through the food-chain of pAmpC, as well as the high frequency of unrelated colistin-resistant isolates is reported.
KW - CTX-M-55
KW - Colistin resistance
KW - Extended-spectrum β-lactamases
KW - Food microbiology
KW - mcr-1
KW - pAmpC
UR - http://www.scopus.com/inward/record.url?scp=85177594604&partnerID=8YFLogxK
U2 - 10.1007/s10123-023-00445-y
DO - 10.1007/s10123-023-00445-y
M3 - Artículo
AN - SCOPUS:85177594604
SN - 1139-6709
JO - International Microbiology
JF - International Microbiology
ER -