TY - JOUR
T1 - The genus Allochromatium (Chromatiales Chromatiaceae) revisited
T2 - A study on its intragenic structure based on multilocus sequence analysis (MLSA) and DNA-DNA hybridization (DDH)
AU - Serrano, Wilbert
AU - Amann, Rudolf
AU - Rosselló-Móra, Ramon
AU - Herbert, Rodney A.
AU - Fischer, Ulrich
N1 - Funding Information:
Support of the Bremen University and the Max Planck Society is gratefully acknowledged. WS was a member of the International Max Planck Research School of Marine Microbiology (MarMic). RRM acknowledges the support of project CLG2009_12651-C02-02 of the Spanish Ministry of Science and Innovation , also co-financed with FEDER support from the European Union .
PY - 2011/12
Y1 - 2011/12
N2 - In this study, the taxonomic status of anoxygenic photosynthetic bacteria belonging to the genus Allochromatium is revisited. The inter- and intraspecies relationship of seven Allochromatium strains, including a set of well described type strains, were examined by DNA-DNA hybridization (DDH) and multilocus sequence analysis (MLSA) using segments of seven protein-coding genes. The re-sequencing of the 16S rRNA, the internal transcriber spacer (ITS), multi-gene analysis and DDH comparison indicated that both type strains Allochromatium vinosum DSM 180 T and Allochromatium minutissimum DSM 1376 T are closely related to each other forming an independent cluster together with the strains A. vinosum DSM 183 and DSM 1686. The internal comparison of members of this A. vinosum phylogroup showed values of DDH relatedness above 80% and concatenated sequence similarities (4744bp) above 98%. In contrast, the MLSA scheme has identified A. vinosum strain BH-2 as a separate lineage. Strain BH-2 was first classified as a member of the species A. vinosum based on DDH comparison. However, this strain showed the lowest similarity values of the 16S rRNA gene and concatenated sequences, as well as amino acid identity (AAI) when compared to other Allochromatium strains, suggesting that strain BH-2 may represent a new species.
AB - In this study, the taxonomic status of anoxygenic photosynthetic bacteria belonging to the genus Allochromatium is revisited. The inter- and intraspecies relationship of seven Allochromatium strains, including a set of well described type strains, were examined by DNA-DNA hybridization (DDH) and multilocus sequence analysis (MLSA) using segments of seven protein-coding genes. The re-sequencing of the 16S rRNA, the internal transcriber spacer (ITS), multi-gene analysis and DDH comparison indicated that both type strains Allochromatium vinosum DSM 180 T and Allochromatium minutissimum DSM 1376 T are closely related to each other forming an independent cluster together with the strains A. vinosum DSM 183 and DSM 1686. The internal comparison of members of this A. vinosum phylogroup showed values of DDH relatedness above 80% and concatenated sequence similarities (4744bp) above 98%. In contrast, the MLSA scheme has identified A. vinosum strain BH-2 as a separate lineage. Strain BH-2 was first classified as a member of the species A. vinosum based on DDH comparison. However, this strain showed the lowest similarity values of the 16S rRNA gene and concatenated sequences, as well as amino acid identity (AAI) when compared to other Allochromatium strains, suggesting that strain BH-2 may represent a new species.
KW - Allochromatium
KW - Classification
KW - DNA-DNA hybridization
KW - Multilocus sequence analysis
UR - http://www.scopus.com/inward/record.url?scp=83155180489&partnerID=8YFLogxK
U2 - 10.1016/j.syapm.2011.06.002
DO - 10.1016/j.syapm.2011.06.002
M3 - Artículo
C2 - 21885227
AN - SCOPUS:83155180489
SN - 0723-2020
VL - 34
SP - 590
EP - 594
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 8
ER -