TY - JOUR
T1 - RESISTENCIA A CEFALOSPORINAS Y QUINOLONAS EN Escherichia coli AISLADAS DE AGUA DE RIEGO DEL RÍO RÍMAC EN LIMA ESTE, PERÚ
AU - Iturrizaga, Mónica Huamán
AU - Salvador-Luján, Gina
AU - Morales, Liliana
AU - Luna, Jeanne Alba
AU - Garcia, Lino Velasquez
AU - Perez, Julio Daniel Pacheco
AU - Pons, Maria J.
N1 - Publisher Copyright:
© 2024, Revista Peruana de Medicina Experimental y Salud Pública.
PY - 2024/4/1
Y1 - 2024/4/1
N2 - Objetives. To evaluate the presence and sensitivity to antimicrobials of Escherichia coli strains isolated from 24 irrigation water samples from the Rimac river of East Lima, Peru. Materials and methods. The E. coli strains were identified by PCR. Antibiotic susceptibility was processed by the disk diffusion method. Genes involved in extended spectrum beta-lactamases (BLEE), quinolones and virulence were determined by PCR. Results. All samples exceeded the acceptable limits established in the Environmental Quality Standards for vegetable irrigation. Of the 94 isolates, 72.3% showed resistance to at least one antibiotic, 24.5% were multidrug resistant (MDR) and 2.1% were extremely resistant. The highest percentages of resistance were observed for ampicillin-sulbactam (57.1%), nalidixic acid (50%), trimethoprim-sulfamethoxazole (35.5%) and ciprofloxacin (20.4%). Among the isolates, 3.2% had a BLEE phenotype related to the bla CTX-M-15 gene. qnrB (20.4%) was the most frequent transferable mechanism of resistance to quinolones, and 2.04% had qnrS. It was estimated that 5.3% were diarrheagenic E. coli and of these, 60% were enterotoxigenic E. coli, 20% were enteropathogenic E. coli and 20% were enteroaggregative E. coli. Conclusions. The results show the existence of diarrheogenic pathotypes in the water used for irrigation of fresh produce and highlight the presence of BLEE-and MDR-producing E. coli, demonstrating the role played by irrigation water in the dissemination of resistance genes in Peru.
AB - Objetives. To evaluate the presence and sensitivity to antimicrobials of Escherichia coli strains isolated from 24 irrigation water samples from the Rimac river of East Lima, Peru. Materials and methods. The E. coli strains were identified by PCR. Antibiotic susceptibility was processed by the disk diffusion method. Genes involved in extended spectrum beta-lactamases (BLEE), quinolones and virulence were determined by PCR. Results. All samples exceeded the acceptable limits established in the Environmental Quality Standards for vegetable irrigation. Of the 94 isolates, 72.3% showed resistance to at least one antibiotic, 24.5% were multidrug resistant (MDR) and 2.1% were extremely resistant. The highest percentages of resistance were observed for ampicillin-sulbactam (57.1%), nalidixic acid (50%), trimethoprim-sulfamethoxazole (35.5%) and ciprofloxacin (20.4%). Among the isolates, 3.2% had a BLEE phenotype related to the bla CTX-M-15 gene. qnrB (20.4%) was the most frequent transferable mechanism of resistance to quinolones, and 2.04% had qnrS. It was estimated that 5.3% were diarrheagenic E. coli and of these, 60% were enterotoxigenic E. coli, 20% were enteropathogenic E. coli and 20% were enteroaggregative E. coli. Conclusions. The results show the existence of diarrheogenic pathotypes in the water used for irrigation of fresh produce and highlight the presence of BLEE-and MDR-producing E. coli, demonstrating the role played by irrigation water in the dissemination of resistance genes in Peru.
KW - ESBL-producers
KW - Escherichia coli
KW - antibiotic resistance
KW - diarrhoeagenic E. coli
KW - irrigation water
UR - http://www.scopus.com/inward/record.url?scp=85202003783&partnerID=8YFLogxK
U2 - 10.17843/rpmesp.2024.412.13246
DO - 10.17843/rpmesp.2024.412.13246
M3 - Artículo
C2 - 39166633
AN - SCOPUS:85202003783
SN - 1726-4634
VL - 41
SP - 114
EP - 120
JO - Revista Peruana de Medicina Experimental y Salud Publica
JF - Revista Peruana de Medicina Experimental y Salud Publica
IS - 2
ER -