TY - JOUR
T1 - Presencia de enterobacteriaceae y escherichia coli multirresistente a antimicrobianos en carne adquirida en mercados tradicionales en lima
AU - Ruiz-Roldán, Lidia
AU - Martínez-Puchol, Sandra
AU - Gomes, Cláudia
AU - Palma, Noemí
AU - Riveros, Maribel
AU - Ocampo, Karen
AU - Durand, David
AU - Ochoa, Theresa J.
AU - Ruiz, Joaquim
AU - Pons, Maria J.
N1 - Publisher Copyright:
© 2018, Instituto Nacional de Salud. All rights reserved.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Objective. The objective of this study was to describe the presence of Enterobacteriaceae in meat samples collected in traditional markets of Lima and to establish the levels of antimicrobial resistance and the presence of extended spectrum beta-lactamases (BLEE) and AmpC in Escherichia coli. Materials and Methods. A total of 138 meat samples, 64 (46.4%) chicken, 44 (31.9%) beef and 30 (21.7%) pork were collected. The isolated bacteria belonged to 17 different genera and, specifically, 14 were classified as Enterobacteriaceae. Sensitivity to ten antimicrobial agents was analyzed using the Kirby-Bauer disc diffusion method, BLEE and AmpC were determined by double disc and imipenem-ceftazidime induction tests, respectively. Results. Antimicrobial resistance levels were high against trimethoprim-sulfamethoxazole, ampicillin, tetracycline, nalidixic acid, ciprofloxacin and chloramphenicol. There are significant differences in antibiotic resistance levels depending on the type of meat (chicken, beef and pork) (p <0.05). Multiple drug resistance (MDR) levels were particularly high in chicken and pork (98.2% and 86.4%, respectively). In addition, the presence of BLEE in Escherichia coli isolated from chicken meat was 59.4%. Conclusions. Multiple drug resistance levels were high compared to antibiotics frequently used in humans; chicken and beef are highlighted as potential reservoirs of BLEE and pAmpC-producing Escherichia coli, respectively.
AB - Objective. The objective of this study was to describe the presence of Enterobacteriaceae in meat samples collected in traditional markets of Lima and to establish the levels of antimicrobial resistance and the presence of extended spectrum beta-lactamases (BLEE) and AmpC in Escherichia coli. Materials and Methods. A total of 138 meat samples, 64 (46.4%) chicken, 44 (31.9%) beef and 30 (21.7%) pork were collected. The isolated bacteria belonged to 17 different genera and, specifically, 14 were classified as Enterobacteriaceae. Sensitivity to ten antimicrobial agents was analyzed using the Kirby-Bauer disc diffusion method, BLEE and AmpC were determined by double disc and imipenem-ceftazidime induction tests, respectively. Results. Antimicrobial resistance levels were high against trimethoprim-sulfamethoxazole, ampicillin, tetracycline, nalidixic acid, ciprofloxacin and chloramphenicol. There are significant differences in antibiotic resistance levels depending on the type of meat (chicken, beef and pork) (p <0.05). Multiple drug resistance (MDR) levels were particularly high in chicken and pork (98.2% and 86.4%, respectively). In addition, the presence of BLEE in Escherichia coli isolated from chicken meat was 59.4%. Conclusions. Multiple drug resistance levels were high compared to antibiotics frequently used in humans; chicken and beef are highlighted as potential reservoirs of BLEE and pAmpC-producing Escherichia coli, respectively.
KW - Escherichia coli
KW - Food
KW - Latin America (source: MeSH NLM)
KW - Meat
KW - Multidrug resistance
KW - Perú
KW - Resistance to beta-lactamases
UR - http://www.scopus.com/inward/record.url?scp=85059608155&partnerID=8YFLogxK
U2 - 10.17843/rpmesp.2018.353.3737
DO - 10.17843/rpmesp.2018.353.3737
M3 - Artículo
C2 - 30517502
AN - SCOPUS:85059608155
SN - 1726-4634
VL - 35
SP - 425
EP - 432
JO - Revista Peruana de Medicina Experimental y Salud Publica
JF - Revista Peruana de Medicina Experimental y Salud Publica
IS - 3
ER -