TY - JOUR
T1 - Partial characterization of a transposon containing the tet(A) determinant in a clinical isolate of Acinetobacter baumannii
AU - Ribera, Anna
AU - Roca, Ignasi
AU - Ruiz, Joaquim
AU - Gibert, Isidre
AU - Vila, Jordi
N1 - Funding Information:
This study was supported by grants from Fondo de Investigaciones Sanitarias (FIS 00/0997), Spain; the Spanish Dirección General de Enseñanza Superior e Investigación Científica (PB97–0196), and partially by Fundació Maria Francesca de Roviralta, Spain. A.R. has a fellowship from the Ministerio de Educación y Ciencia, Spain and I.R. has a predoctoral fellowship from the Direcció General d’Uni-versitats de la Generalitat de Catalunya.
PY - 2003/9/1
Y1 - 2003/9/1
N2 - A genomic library from one clinical isolate of Acinetobacter baumannii was obtained to find genes responsible for tetracycline resistance. Escherichia coli DH5α-MDR transformants, selected on Mueller-Hinton agar supplemented with tetracycline 18 mg/L, were thoroughly characterized. In one clone, with an insert of 7070 bp, it was found that the resistance to tetracycline was mediated by the tet(A) gene (1200 bp) which encodes a tetracycline efflux pump. This gene was recovered together with tetR(A) (651 bp), the tet(A) repressor. Moreover, the partial sequence (2008 bp) of a transposase gene, tnpA, and 1316 bp corresponding to an IS, similar to that described in one strain of Salmonella typhi(IS4321), were found. In this A. baumannii clinical isolate, the tet(A) gene is located in a transposon. The structure of this transposon is similar to that of Tn1721, with the tet(A), tetR(A) and the regions between these genes, being closely related to those of Tn1721. The data indicate horizontal transfer of tetracycline resistance genes between A. baumannii and other genera sharing the same ecological niches.
AB - A genomic library from one clinical isolate of Acinetobacter baumannii was obtained to find genes responsible for tetracycline resistance. Escherichia coli DH5α-MDR transformants, selected on Mueller-Hinton agar supplemented with tetracycline 18 mg/L, were thoroughly characterized. In one clone, with an insert of 7070 bp, it was found that the resistance to tetracycline was mediated by the tet(A) gene (1200 bp) which encodes a tetracycline efflux pump. This gene was recovered together with tetR(A) (651 bp), the tet(A) repressor. Moreover, the partial sequence (2008 bp) of a transposase gene, tnpA, and 1316 bp corresponding to an IS, similar to that described in one strain of Salmonella typhi(IS4321), were found. In this A. baumannii clinical isolate, the tet(A) gene is located in a transposon. The structure of this transposon is similar to that of Tn1721, with the tet(A), tetR(A) and the regions between these genes, being closely related to those of Tn1721. The data indicate horizontal transfer of tetracycline resistance genes between A. baumannii and other genera sharing the same ecological niches.
KW - Acinetobacter baumannii
KW - Tetracycline resistance
UR - http://www.scopus.com/inward/record.url?scp=0141852787&partnerID=8YFLogxK
U2 - 10.1093/jac/dkg344
DO - 10.1093/jac/dkg344
M3 - Artículo
C2 - 12888597
AN - SCOPUS:0141852787
SN - 0305-7453
VL - 52
SP - 477
EP - 480
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
IS - 3
ER -