TY - JOUR
T1 - Molecular typing of Staphylococcus aureus clinical isolates by pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec type determination and dissemination of antibiotic resistance genes
AU - Laplana, Leticia Millán
AU - Cepero, Ma Pilar Goñi
AU - Ruiz, Joaquim
AU - Zolezzi, Paula Cerdá
AU - Calvo, Ma Carmen Rubio
AU - Erazo, Melisa Canales
AU - Gómez-Lus, Rafael
N1 - Funding Information:
This study was supported by Project FIS PI05/2310 (Ministerio de Sanidad y Consumo, Instituto Aragonés de Ciencias de la Salud, Spain) and Project B24 (DGA/GRUPOS CONSOLIDADOS, Departamento de Ciencia, Tecnología y Universidad, Diputación General de Aragón, Spain). L. Millán Laplana was the recipient of fellowship B011/2001 from Diputación General de Aragón, Departamento de Educación y Ciencia, Spain.
PY - 2007/12
Y1 - 2007/12
N2 - Pulsed-field gel electrophoresis (PFGE) of SmaI macrorestriction fragments of genomic DNA as well as staphylococcal cassette chromosome mec (SCCmec) typing for mecA-carrying isolates were used to study the distribution of clonal types among 177 Staphylococcus aureus clinical isolates recovered in a Spanish hospital between 2000 and 2003. Five major clonal types (P1 to P5) were identified by PFGE, with one of them (P1) comprising the majority of strains (47.5%). According to SCCmec typing, SCCmec type IVA was the most prevalent type, showing increasing prevalence in the hospital setting with respect to other pandemic clones. One SCCmec pattern was detected in different PFGE types, which demonstrates that the latter is a major discriminative typing method. Three novel SCCmec elements or variants were found, each in a different PFGE type. Oxacillin (methicillin)-resistant and -susceptible S. aureus (MRSA and MSSA, respectively) strains were detected showing identical PFGE patterns, suggesting horizontal transfer of mecA to MSSA and/or mecA deletion from MRSA. Persistence of several S. aureus clones throughout the years within the same hospital environment was also observed.
AB - Pulsed-field gel electrophoresis (PFGE) of SmaI macrorestriction fragments of genomic DNA as well as staphylococcal cassette chromosome mec (SCCmec) typing for mecA-carrying isolates were used to study the distribution of clonal types among 177 Staphylococcus aureus clinical isolates recovered in a Spanish hospital between 2000 and 2003. Five major clonal types (P1 to P5) were identified by PFGE, with one of them (P1) comprising the majority of strains (47.5%). According to SCCmec typing, SCCmec type IVA was the most prevalent type, showing increasing prevalence in the hospital setting with respect to other pandemic clones. One SCCmec pattern was detected in different PFGE types, which demonstrates that the latter is a major discriminative typing method. Three novel SCCmec elements or variants were found, each in a different PFGE type. Oxacillin (methicillin)-resistant and -susceptible S. aureus (MRSA and MSSA, respectively) strains were detected showing identical PFGE patterns, suggesting horizontal transfer of mecA to MSSA and/or mecA deletion from MRSA. Persistence of several S. aureus clones throughout the years within the same hospital environment was also observed.
KW - Antibiotic resistance genes
KW - Antimicrobial susceptibility
KW - PFGE
KW - SCCmec
KW - Staphylococcus aureus
KW - mecA
UR - http://www.scopus.com/inward/record.url?scp=35748956730&partnerID=8YFLogxK
U2 - 10.1016/j.ijantimicag.2007.06.020
DO - 10.1016/j.ijantimicag.2007.06.020
M3 - Artículo
C2 - 17869068
AN - SCOPUS:35748956730
SN - 0924-8579
VL - 30
SP - 505
EP - 513
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
IS - 6
ER -