Molecular typing of Staphylococcus aureus clinical isolates by pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec type determination and dissemination of antibiotic resistance genes

Leticia Millán Laplana, Ma Pilar Goñi Cepero, Joaquim Ruiz, Paula Cerdá Zolezzi, Ma Carmen Rubio Calvo, Melisa Canales Erazo, Rafael Gómez-Lus

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Pulsed-field gel electrophoresis (PFGE) of SmaI macrorestriction fragments of genomic DNA as well as staphylococcal cassette chromosome mec (SCCmec) typing for mecA-carrying isolates were used to study the distribution of clonal types among 177 Staphylococcus aureus clinical isolates recovered in a Spanish hospital between 2000 and 2003. Five major clonal types (P1 to P5) were identified by PFGE, with one of them (P1) comprising the majority of strains (47.5%). According to SCCmec typing, SCCmec type IVA was the most prevalent type, showing increasing prevalence in the hospital setting with respect to other pandemic clones. One SCCmec pattern was detected in different PFGE types, which demonstrates that the latter is a major discriminative typing method. Three novel SCCmec elements or variants were found, each in a different PFGE type. Oxacillin (methicillin)-resistant and -susceptible S. aureus (MRSA and MSSA, respectively) strains were detected showing identical PFGE patterns, suggesting horizontal transfer of mecA to MSSA and/or mecA deletion from MRSA. Persistence of several S. aureus clones throughout the years within the same hospital environment was also observed.

Original languageEnglish
Pages (from-to)505-513
Number of pages9
JournalInternational Journal of Antimicrobial Agents
Volume30
Issue number6
DOIs
StatePublished - Dec 2007

Keywords

  • Antibiotic resistance genes
  • Antimicrobial susceptibility
  • PFGE
  • SCCmec
  • Staphylococcus aureus
  • mecA

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