Molecular and serologic markers of acute dengue infection in naive and flavivirus-vaccinated travelers

Cristina Domingo; Fernando de Ory; Juan Carlos Sanz; Noelia Reyes; Joaquim Gascón; Ole Wichmann; Sabino Puente; Mirjam Schunk; Rogelio López-Vélez; Joaquim Ruiz; Antonio Tenorio

doi:10.1016/j.diagmicrobio.2009.05.004

Molecular and serologic markers of acute dengue infection in naive and flavivirus-vaccinated travelers

Cristina Domingo, Fernando de Ory, Juan Carlos Sanz, Noelia Reyes, Joaquim Gascón, Ole Wichmann, Sabino Puente, Mirjam Schunk, Rogelio López-Vélez, Joaquim Ruiz, Antonio Tenorio

Medicina humana

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A total of 520 European travelers with suspected dengue fever were examined, and acute dengue virus infection was confirmed in 127 of them. Molecular and serological tests for dengue diagnosis, according to their usefulness in the different stages of the disease, were performed. The accuracy of the IgM/IgG ratio and the IgG avidity index was confirmed during the early phase of the illness to discriminate the serologic status (primary versus secondary immune response) of patients who were either naive or previously vaccinated against other flaviviruses. Virologic markers of secondary infection were detected in 11.8% of nonvaccinated infected patients and in 92.6% of yellow fever vaccinated patients. The proper use of these simple methodologies could help in the identification of patients affected with secondary infection, who may be at a higher risk of developing a hemorrhagic disorder.

Original language	English
Pages (from-to)	42-48
Number of pages	7
Journal	Diagnostic Microbiology and Infectious Disease
Volume	65
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2009.05.004
State	Published - Sep 2009

Keywords

Avidity index
Dengue infection
Flavivirus vaccinated
IgM/IgG ratio
Prognostic marker
Secondary flavivirus infection
Serology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2009.05.004

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title = "Molecular and serologic markers of acute dengue infection in naive and flavivirus-vaccinated travelers",

abstract = "A total of 520 European travelers with suspected dengue fever were examined, and acute dengue virus infection was confirmed in 127 of them. Molecular and serological tests for dengue diagnosis, according to their usefulness in the different stages of the disease, were performed. The accuracy of the IgM/IgG ratio and the IgG avidity index was confirmed during the early phase of the illness to discriminate the serologic status (primary versus secondary immune response) of patients who were either naive or previously vaccinated against other flaviviruses. Virologic markers of secondary infection were detected in 11.8% of nonvaccinated infected patients and in 92.6% of yellow fever vaccinated patients. The proper use of these simple methodologies could help in the identification of patients affected with secondary infection, who may be at a higher risk of developing a hemorrhagic disorder.",

keywords = "Avidity index, Dengue infection, Flavivirus vaccinated, IgM/IgG ratio, Prognostic marker, Secondary flavivirus infection, Serology",

author = "Cristina Domingo and {de Ory}, Fernando and Sanz, {Juan Carlos} and Noelia Reyes and Joaquim Gasc{\'o}n and Ole Wichmann and Sabino Puente and Mirjam Schunk and Rogelio L{\'o}pez-V{\'e}lez and Joaquim Ruiz and Antonio Tenorio",

note = "Funding Information: J. Ruiz is the recipient of a grant (CP05/0130) from Fondo de Investigaciones Sanitarias. This investigation received financial support from the Instituto de Salud Carlos III through research project grants MPY1194/02, MPY1285/06, and the Network RICET (C03/04 and RD06/0021).",

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AU - Domingo, Cristina

AU - de Ory, Fernando

AU - Sanz, Juan Carlos

AU - Reyes, Noelia

AU - Gascón, Joaquim

AU - Wichmann, Ole

AU - Puente, Sabino

AU - Schunk, Mirjam

AU - López-Vélez, Rogelio

AU - Ruiz, Joaquim

AU - Tenorio, Antonio

N1 - Funding Information: J. Ruiz is the recipient of a grant (CP05/0130) from Fondo de Investigaciones Sanitarias. This investigation received financial support from the Instituto de Salud Carlos III through research project grants MPY1194/02, MPY1285/06, and the Network RICET (C03/04 and RD06/0021).

PY - 2009/9

Y1 - 2009/9

N2 - A total of 520 European travelers with suspected dengue fever were examined, and acute dengue virus infection was confirmed in 127 of them. Molecular and serological tests for dengue diagnosis, according to their usefulness in the different stages of the disease, were performed. The accuracy of the IgM/IgG ratio and the IgG avidity index was confirmed during the early phase of the illness to discriminate the serologic status (primary versus secondary immune response) of patients who were either naive or previously vaccinated against other flaviviruses. Virologic markers of secondary infection were detected in 11.8% of nonvaccinated infected patients and in 92.6% of yellow fever vaccinated patients. The proper use of these simple methodologies could help in the identification of patients affected with secondary infection, who may be at a higher risk of developing a hemorrhagic disorder.

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KW - Dengue infection

KW - Flavivirus vaccinated

KW - IgM/IgG ratio

KW - Prognostic marker

KW - Secondary flavivirus infection

KW - Serology

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Molecular and serologic markers of acute dengue infection in naive and flavivirus-vaccinated travelers

Abstract

Keywords

UN SDGs

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