Abstract
The mechanism of quinolone resistance in 42 food-borne strains of Salmonella typhimurium which showed nalidixic acid resistance was investigated. Analysis of outer membrane proteins and lipopolysaccharides from both quinolone-susceptible and resistant did not shown any differences among the clinical isolates tested. A 285 bp PCR fragment containing the quinolone resistance determining region of the gyrA gene and a 237 bp PCR fragment containing the region of parc homologous to the quinolone- resistance determining region of the gyrA gene, were sequenced. All the resistant strains presented a change at Ser-83 to Phe in the GyrA protein, but no changes were observed in the ParC protein. These findings indicate that this mutation in gyrA gene plays a major role in the acquisition of nalidixic acid resistance in clinical isolates of S. typhimurium.
| Translated title of the contribution | Quinolone resistance mechanism in Salmonella typhimurium strains |
|---|---|
| Original language | Spanish |
| Pages (from-to) | 63-69 |
| Number of pages | 7 |
| Journal | Ars Pharmaceutica |
| Volume | 38 |
| Issue number | 1 |
| State | Published - 1997 |