Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila

Gustavo S. Claudiano, Jefferson Yunis-Aguinaga, Fausto A. Marinho-Neto, Renata L. Miranda, Isabela M. Martins, Fabrizia S. Otani, Antonio V. Mundim, Cleni M. Marzocchi-Machado, Julieta R.E. Moraes, Flávio Ruas de Moraes

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The pathogenesis of sepsis involves complex systems and multiple interrelationships between the host and pathogen producing high mortality rates in various animal species. In this study, hematological disturbances, innate immunity and survival during the septic process in Piaractus mesopotamicus inoculated with Aeromonas hydrophila were studied. For this aim, fish blood samples were taken from control and infected groups 1, 3, 6, and 9 h post-inoculation (HPI). Leukogram showed reduction in the number of leukocytes and thrombocytes, followed by cessation of leukocyte chemotaxis 6 HPI and severe morphological changes in leukocytes and erythrocytes. At 3 HPI production of reactive oxygen species increased and at 6 HPI decreased. There was no change in serum lysozyme concentration and lytic activity of the complement system, despite the progressive increase in serum lytic activity and bacterial agglutination. Finally, the changes in clinical signs due to aeromonosis and increasing septicemia resulted in a reduction in survival to 57.14% after 36 HPI. It was possible concluded that these hematological and immune are crucial event in the worsening of sepsis in P. mesopotamicus, and these findings are utility for diagnosing and understanding the pathophysiology sepsis in pacu induced by A. hydrophila.

Original languageEnglish
Pages (from-to)259-265
Number of pages7
JournalFish and Shellfish Immunology
Volume88
DOIs
StatePublished - May 2019

Keywords

  • Bacteria
  • Blood
  • Innate immunity
  • Leukocytes
  • Pacu

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