Evaluación de dimetilacetamida y dimetilformamida como agentes crioprotectores para espermatozoides epididimarios de alpaca (Vicugna pacos)

Translated title of the contribution: Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)

Winnie Contreras, Luis Ruiz García, Alexei Santiani

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The aim of this study was to determine the effect of the type (dimethylacetamide [DMA] and dimethylformamide [DMF]) and concentrations (1, 3.5, 7%) of cryoprotective agents on three sperm parameters during the cryopreservation process of epididymal alpaca sperm. A 2x3 factorial design consisting of the two cryoprotectants and the three concentrations was used. The parameters of sperm viability (SYBR-14/PI) and mitochondrial membrane potential (MitoTracker Deep Red FM) of sperm of 40 epididymis were evaluated by flow cytometry and motility by microscopy after thawing. A higher percentage of motility after thawing was found with AMD (14%) than with DMF (10%) (p=0.001). In addition, there was a significant effect of the cryoprotectant concentration in all the parameters evaluated (p<0.001), being better at concentrations of 1 and 3.5% than with 7%. No significant effect of the cryoprotectant-concentration interaction was found.

Translated title of the contributionEvaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
Original languageSpanish
Article numbere17548
JournalRevista de Investigaciones Veterinarias del Peru
Volume31
Issue number1
DOIs
StatePublished - 29 Mar 2020
Externally publishedYes

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