Effects of a triple antibiotic solution on pulpal dynamics after intentionally delayed tooth replantation in mice

Angela Quispe-Salcedo, Hiroko Ida-Yonemochi, Hayato Ohshima

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Introduction This study analyzed the detailed biological events underlying pulpal dynamics evoked by 3Mix (the mixture of ciprofloxacin, metronidazole, and minocycline) solution after intentionally delayed tooth replantation because 3Mix improves pulpal healing after tooth injuries.

Methods The maxillary first molars of 3-week-old mice were extracted and immersed in 3Mix solution for 30 minutes in comparison with phosphate buffered saline (PBS) alone. Cell proliferation, apoptosis, and differentiation were assessed in extracted/replanted teeth during days 0-14 using immunohistochemistry, apoptosis assay, and reverse-transcriptase polymerase chain reaction.

Conclusions The results suggest that the application of 3Mix may suppress osteoblast differentiation by the migration of dendritic cells to the injury site and via the activation of stem/progenitor cells, resulting in the acceleration of odontoblastlike cell differentiation.

Results 3Mix solution accelerated odontoblast differentiation in the coronal pulp on day 7 and tertiary dentin formation on day 14, whereas the regenerative process was delayed in the PBS group. Cell proliferation and apoptosis occurred in the pulp of the 3Mix group during days 5-7 and subsequently decreased from days 7-14. On day 5, dentin sialophosphoprotein and nestin were first recovered in the 3Mix group, whereas expression levels for alkaline phosphatase, osteopontin, and osteocalcin increased in the PBS group. The expression levels for octamer-binding factor 3/4A and 3/4B reached the maximum level on day 1 and were sharply decreased on day 3 in both groups. High expression levels of Cd11c were first observed in the 3Mix group on day 1 and later at days 5 and 7.

Original languageEnglish
Pages (from-to)1566-1572
Number of pages7
JournalJournal of Endodontics
Issue number10
StatePublished - 1 Oct 2014


  • Antimicrobial
  • Crlj:CD1 (ICR) mice
  • apoptosis
  • cell differentiation
  • cell proliferation
  • dental pulp
  • odontoblasts
  • regeneration
  • tooth replantation


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