Feeding stress or suboptimal feeding in fish can have a direct impact on their energy metabolism and feed use efficiency, and thus on productivity. That impact can be quantified objectively by comparing the expression of genes between fish fed ad libitum and fish fed suboptimally. Moreover, some of the genes expressed in animals fed suboptimally can also be used as candidate genes to identify feeding stress in general. To identify some of those genes, a group of 60 tilapia Oreochromis nilo ticus (L., 1758) was divided into two batches (fed ad libitum or suboptimally for 22 days) with two replicates. For this purpose, we carried out differential display analysis in brain tissue samples using 36 combinations o 3 polyT anchoring primers and 12 random primers. An average of 25 bands was produced per primer pair, with 31 bands expressed differentially which were excised from, the gels, sequenced, and annotated with information available on public databases. Some sequences matched genes with different binding activities, regulation of transcription, and those playing a role in synapses. One of the most interesting findings was the ALOXC5 gene, which plays a role in the production of lukotrienes, important mediators in inflammatory processes. The identification of genes corresponding to those bands will provide more information about mechanisms that play a role in stressful situations produced by suboptimal feeding, as well as other causes.
|Translated title of the contribution||Differential gene expression in tilapia Oreochromis niloticus (L., 1758) under feeding stress|
|Number of pages||10|
|Journal||Boletin - Instituto Espanol de Oceanografia|
|State||Published - 2005|