TY - JOUR
T1 - Diarrhoea caused by rotavirus in a regional Peruvian hospital
T2 - Determination of circulating genotypes
AU - Espejo, Pablo Weilg
AU - Peralta, Fiorella Orellana
AU - Pacheres, Hernán Cornejo
AU - Del Valle, Luis J.
AU - Tapia, Angela Cornejo
AU - Mayra, Jorge Bazán
AU - Ruiz, Joaquim
AU - Mendoza, Juana del Valle
N1 - Funding Information:
Funding: This work was supported by the 1st Concurso Incentivo a la Investigación de la Universidad Peruana de Ciencias Aplicadas (UPC) (Lima, Peru). JR has a fellowship from the program I3 of the ISCIII [grant no. CES11/012].
PY - 2014/7
Y1 - 2014/7
N2 - Background: Gastroenteritis caused by rotavirus is responsible for approximately 810 deaths per year in children under 5 years of age in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular epidemiology of rotavirus diarrhoea in Peru. Methods: A total of 131 stool samples were obtained from children under 5 years hospitalised from January 2010 to December 2012 in the Hospital Regional de Cajamarca (Peru). ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were performed by semi-nested multiplex RT-PCR, and sequencing was performed to confirm the PCR results. Results: Of the 117 samples available, 22 (18.8%) tested positive for rotavirus by ELISA and 42 (35.9%) tested positive by RT-PCR. Among the G genotypes identified, G9 (35.7%; 15/42) and G12 (33.3%; 14/42) were the most prevalent, with the most common combination being G12/P[6] (23.8%; 10/42). Conclusions: A high prevalence of the G12/P[6] genotype was detected. It is known that this genotype is not covered by the current vaccines available. More in-depth studies are needed to determine the current rotavirus genotypes presents in Peru.
AB - Background: Gastroenteritis caused by rotavirus is responsible for approximately 810 deaths per year in children under 5 years of age in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular epidemiology of rotavirus diarrhoea in Peru. Methods: A total of 131 stool samples were obtained from children under 5 years hospitalised from January 2010 to December 2012 in the Hospital Regional de Cajamarca (Peru). ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were performed by semi-nested multiplex RT-PCR, and sequencing was performed to confirm the PCR results. Results: Of the 117 samples available, 22 (18.8%) tested positive for rotavirus by ELISA and 42 (35.9%) tested positive by RT-PCR. Among the G genotypes identified, G9 (35.7%; 15/42) and G12 (33.3%; 14/42) were the most prevalent, with the most common combination being G12/P[6] (23.8%; 10/42). Conclusions: A high prevalence of the G12/P[6] genotype was detected. It is known that this genotype is not covered by the current vaccines available. More in-depth studies are needed to determine the current rotavirus genotypes presents in Peru.
KW - Acute gastroenteritis
KW - Epidemiology
KW - Peru
KW - Rotavirus
KW - Viral genotypes
UR - http://www.scopus.com/inward/record.url?scp=84902506907&partnerID=8YFLogxK
U2 - 10.1093/trstmh/tru059
DO - 10.1093/trstmh/tru059
M3 - Artículo
C2 - 24778205
AN - SCOPUS:84902506907
SN - 0035-9203
VL - 108
SP - 425
EP - 430
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
IS - 7
M1 - tru059
ER -
