Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains

Theresa J. Ochoa, Fulton P. Rivera, Maria Bernal, Rina Meza, Lucie Ecker, Ana I. Gil, David Cepeda, Susan Mosquito, Erik Mercado, Ryan C. Maves, Eric R. Hall, Ann Mari Svennerholm, Annette McVeigh, Stephen Savarino, Claudio F. Lanata

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


We analyzed a randomly selected group of 30 diffusely adherent (DAEC), 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli (ETEC) colonization factors (CFs) were evaluated by a dot-blot assay using 21 CF-specific monoclonal antibodies. Out of 95 non-ETEC strains, three DAEC were found to express coli surface antigen 20 (CS20). No other E. coli expressed CFs. We confirmed the three CS20-positive strains as ETEC-negative by repeat PCR and as toxin-negative by ganglioside-GM1-enzyme-linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non-ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli, which may then play a role in pathogenesis and the immune response of the host. FEMS Immunology & Medical Microbiology

Original languageEnglish
Pages (from-to)186-189
Number of pages4
JournalFEMS Immunology and Medical Microbiology
Issue number2
StatePublished - Nov 2010


  • Children
  • Colonization factor antigens
  • Diarrhea
  • Diarrheagenic Escherichia coli
  • Diffusely adherent Escherichia coli
  • Enterotoxigenic Escherichia coli


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