Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains

Theresa J. Ochoa; Fulton P. Rivera; Maria Bernal; Rina Meza; Lucie Ecker; Ana I. Gil; David Cepeda; Susan Mosquito; Erik Mercado; Ryan C. Maves; Eric R. Hall; Ann Mari Svennerholm; Annette McVeigh; Stephen Savarino; Claudio F. Lanata

doi:10.1111/j.1574-695X.2010.00730.x

Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains

Theresa J. Ochoa, Fulton P. Rivera, Maria Bernal, Rina Meza, Lucie Ecker, Ana I. Gil, David Cepeda, Susan Mosquito, Erik Mercado, Ryan C. Maves, Eric R. Hall, Ann Mari Svennerholm, Annette McVeigh, Stephen Savarino, Claudio F. Lanata

Medicina humana

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We analyzed a randomly selected group of 30 diffusely adherent (DAEC), 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli (ETEC) colonization factors (CFs) were evaluated by a dot-blot assay using 21 CF-specific monoclonal antibodies. Out of 95 non-ETEC strains, three DAEC were found to express coli surface antigen 20 (CS20). No other E. coli expressed CFs. We confirmed the three CS20-positive strains as ETEC-negative by repeat PCR and as toxin-negative by ganglioside-GM1-enzyme-linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non-ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli, which may then play a role in pathogenesis and the immune response of the host. FEMS Immunology & Medical Microbiology

Original language	English
Pages (from-to)	186-189
Number of pages	4
Journal	FEMS Immunology and Medical Microbiology
Volume	60
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-695X.2010.00730.x
State	Published - Nov 2010

Keywords

Children
Colonization factor antigens
Diarrhea
Diarrheagenic Escherichia coli
Diffusely adherent Escherichia coli
Enterotoxigenic Escherichia coli

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1574-695X.2010.00730.x

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Ochoa, T. J., Rivera, F. P., Bernal, M., Meza, R., Ecker, L., Gil, A. I., Cepeda, D., Mosquito, S., Mercado, E., Maves, R. C., Hall, E. R., Svennerholm, A. M., McVeigh, A., Savarino, S., & Lanata, C. F. (2010). Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains. FEMS Immunology and Medical Microbiology, 60(2), 186-189. https://doi.org/10.1111/j.1574-695X.2010.00730.x

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title = "Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains",

abstract = "We analyzed a randomly selected group of 30 diffusely adherent (DAEC), 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli (ETEC) colonization factors (CFs) were evaluated by a dot-blot assay using 21 CF-specific monoclonal antibodies. Out of 95 non-ETEC strains, three DAEC were found to express coli surface antigen 20 (CS20). No other E. coli expressed CFs. We confirmed the three CS20-positive strains as ETEC-negative by repeat PCR and as toxin-negative by ganglioside-GM1-enzyme-linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non-ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli, which may then play a role in pathogenesis and the immune response of the host. FEMS Immunology & Medical Microbiology",

keywords = "Children, Colonization factor antigens, Diarrhea, Diarrheagenic Escherichia coli, Diffusely adherent Escherichia coli, Enterotoxigenic Escherichia coli",

author = "Ochoa, {Theresa J.} and Rivera, {Fulton P.} and Maria Bernal and Rina Meza and Lucie Ecker and Gil, {Ana I.} and David Cepeda and Susan Mosquito and Erik Mercado and Maves, {Ryan C.} and Hall, {Eric R.} and Svennerholm, {Ann Mari} and Annette McVeigh and Stephen Savarino and Lanata, {Claudio F.}",

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Ochoa, TJ, Rivera, FP, Bernal, M, Meza, R, Ecker, L, Gil, AI, Cepeda, D, Mosquito, S, Mercado, E, Maves, RC, Hall, ER, Svennerholm, AM, McVeigh, A, Savarino, S & Lanata, CF 2010, 'Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains', FEMS Immunology and Medical Microbiology, vol. 60, no. 2, pp. 186-189. https://doi.org/10.1111/j.1574-695X.2010.00730.x

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T1 - Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains

AU - Ochoa, Theresa J.

AU - Rivera, Fulton P.

AU - Bernal, Maria

AU - Meza, Rina

AU - Ecker, Lucie

AU - Gil, Ana I.

AU - Cepeda, David

AU - Mosquito, Susan

AU - Mercado, Erik

AU - Maves, Ryan C.

AU - Hall, Eric R.

AU - Svennerholm, Ann Mari

AU - McVeigh, Annette

AU - Savarino, Stephen

AU - Lanata, Claudio F.

PY - 2010/11

Y1 - 2010/11

N2 - We analyzed a randomly selected group of 30 diffusely adherent (DAEC), 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli (ETEC) colonization factors (CFs) were evaluated by a dot-blot assay using 21 CF-specific monoclonal antibodies. Out of 95 non-ETEC strains, three DAEC were found to express coli surface antigen 20 (CS20). No other E. coli expressed CFs. We confirmed the three CS20-positive strains as ETEC-negative by repeat PCR and as toxin-negative by ganglioside-GM1-enzyme-linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non-ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli, which may then play a role in pathogenesis and the immune response of the host. FEMS Immunology & Medical Microbiology

AB - We analyzed a randomly selected group of 30 diffusely adherent (DAEC), 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli (ETEC) colonization factors (CFs) were evaluated by a dot-blot assay using 21 CF-specific monoclonal antibodies. Out of 95 non-ETEC strains, three DAEC were found to express coli surface antigen 20 (CS20). No other E. coli expressed CFs. We confirmed the three CS20-positive strains as ETEC-negative by repeat PCR and as toxin-negative by ganglioside-GM1-enzyme-linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non-ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli, which may then play a role in pathogenesis and the immune response of the host. FEMS Immunology & Medical Microbiology

KW - Children

KW - Colonization factor antigens

KW - Diarrhea

KW - Diarrheagenic Escherichia coli

KW - Diffusely adherent Escherichia coli

KW - Enterotoxigenic Escherichia coli

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DO - 10.1111/j.1574-695X.2010.00730.x

M3 - Artículo

C2 - 21064230

AN - SCOPUS:78650408676

SN - 0928-8244

VL - 60

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JO - FEMS Immunology and Medical Microbiology

JF - FEMS Immunology and Medical Microbiology

IS - 2

ER -

Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains

Abstract

Keywords

UN SDGs

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