Detection of mutations in parC in quinolone-resistant clinical isolates of Escherichia coli

Jordi Vila, Joaquim Ruiz, Pilar Goñi, M. Teresa Jimenez De Anta

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Abstract

The gene parC encodes the A subunit of topoisomerase IV of Escherichia coli. Mutations in the parC region analogous to those in the quinolone resistance-determining region of gyrA were investigated in 27 clinical isolates of E. coli for which ciprofloxacin MICs were 0.007 to 128 μ/ml. Of 15 isolates for which ciprofloxacin MICs were ≥1 μg/ml, 8 showed a change in the serine residue at position 80 (Ser-80), 4 showed a change in Glu-84, and 3 showed changes in both amino acids. No mutations were detected in 12 clinical isolates for which ciprofloxacin MICs were ≤0.25 μg/ml. These findings suggest that Parc from E. coli may be another target for quinolones and that mutations at residues Ser-80 and Glu-84 may contribute to decreased fluoroquinolone susceptibility.

Original languageEnglish
Pages (from-to)491-493
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume40
Issue number2
DOIs
StatePublished - Feb 1996

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