Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR

Carlos A Castaneda; Miluska Castillo; Joselyn Sanchez; Sandro Casavilca; Juvenal Sanchez; Luis A Bernabe; Nancy Suarez; Ivan Chavez; Eloy Ruiz; Katherine Tello; Maria R Villa; Rocio Zevallos; Paola Montenegro; Emmanuel Dias-Neto; Maria Landa-Baella; Luis Taxa

doi:10.2217/fmb-2019-0280

Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR

Carlos A Castaneda, Miluska Castillo, Joselyn Sanchez, Sandro Casavilca, Juvenal Sanchez, Luis A Bernabe, Nancy Suarez, Ivan Chavez, Eloy Ruiz, Katherine Tello, Maria R Villa, Rocio Zevallos, Paola Montenegro, Emmanuel Dias-Neto, Maria Landa-Baella, Luis Taxa

Medicina humana

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.

Original language	English
Pages (from-to)	1131-1137
Number of pages	7
Journal	Future Microbiology
Volume	15
Issue number	12
DOIs	https://doi.org/10.2217/fmb-2019-0280
State	Published - Aug 2020

Keywords

Helicobacter pylori
PCR
eosin
hematoxylin
histopathology
immunohistochemistry
stomach neoplasms

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/fmb-2019-0280

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A Castaneda, C., Castillo, M., Sanchez, J., Casavilca, S., Sanchez, J., A Bernabe, L., Suarez, N., Chavez, I., Ruiz, E., Tello, K., R Villa, M., Zevallos, R., Montenegro, P., Dias-Neto, E., Landa-Baella, M., & Taxa, L. (2020). Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR. Future Microbiology, 15(12), 1131-1137. https://doi.org/10.2217/fmb-2019-0280

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title = "Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR",

abstract = "Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.",

keywords = "Helicobacter pylori, PCR, eosin, hematoxylin, histopathology, immunohistochemistry, stomach neoplasms",

author = "{A Castaneda}, Carlos and Miluska Castillo and Joselyn Sanchez and Sandro Casavilca and Juvenal Sanchez and {A Bernabe}, Luis and Nancy Suarez and Ivan Chavez and Eloy Ruiz and Katherine Tello and {R Villa}, Maria and Rocio Zevallos and Paola Montenegro and Emmanuel Dias-Neto and Maria Landa-Baella and Luis Taxa",

year = "2020",

month = aug,

doi = "10.2217/fmb-2019-0280",

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A Castaneda, C, Castillo, M, Sanchez, J, Casavilca, S, Sanchez, J, A Bernabe, L, Suarez, N, Chavez, I, Ruiz, E, Tello, K, R Villa, M, Zevallos, R, Montenegro, P, Dias-Neto, E, Landa-Baella, M & Taxa, L 2020, 'Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR', Future Microbiology, vol. 15, no. 12, pp. 1131-1137. https://doi.org/10.2217/fmb-2019-0280

TY - JOUR

T1 - Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR

AU - A Castaneda, Carlos

AU - Castillo, Miluska

AU - Sanchez, Joselyn

AU - Casavilca, Sandro

AU - Sanchez, Juvenal

AU - A Bernabe, Luis

AU - Suarez, Nancy

AU - Chavez, Ivan

AU - Ruiz, Eloy

AU - Tello, Katherine

AU - R Villa, Maria

AU - Zevallos, Rocio

AU - Montenegro, Paola

AU - Dias-Neto, Emmanuel

AU - Landa-Baella, Maria

AU - Taxa, Luis

PY - 2020/8

Y1 - 2020/8

N2 - Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.

AB - Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.

KW - Helicobacter pylori

KW - PCR

KW - eosin

KW - hematoxylin

KW - histopathology

KW - immunohistochemistry

KW - stomach neoplasms

UR - http://www.scopus.com/inward/record.url?scp=85091266055&partnerID=8YFLogxK

U2 - 10.2217/fmb-2019-0280

DO - 10.2217/fmb-2019-0280

M3 - Artículo

C2 - 32954850

AN - SCOPUS:85091266055

SN - 1746-0913

VL - 15

SP - 1131

EP - 1137

JO - Future Microbiology

JF - Future Microbiology

IS - 12

ER -

Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR

Abstract

Keywords

UN SDGs

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