Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR

Carlos A Castaneda, Miluska Castillo, Joselyn Sanchez, Sandro Casavilca, Juvenal Sanchez, Luis A Bernabe, Nancy Suarez, Ivan Chavez, Eloy Ruiz, Katherine Tello, Maria R Villa, Rocio Zevallos, Paola Montenegro, Emmanuel Dias-Neto, Maria Landa-Baella, Luis Taxa

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.

Original languageEnglish
Pages (from-to)1131-1137
Number of pages7
JournalFuture Microbiology
Volume15
Issue number12
DOIs
StatePublished - Aug 2020

Keywords

  • Helicobacter pylori
  • PCR
  • eosin
  • hematoxylin
  • histopathology
  • immunohistochemistry
  • stomach neoplasms

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