TY - JOUR
T1 - Detection of dihydrofolate reductase genes by PCR and RFLP
AU - Navia, Margarita M.
AU - Ruiz, Joaquim
AU - Sanchez-Cespedes, Javier
AU - Vila, Jordi
N1 - Funding Information:
We thank Dr. S.G.B. Amyes for kindly providing some of the strains. This work was partially supported by grant FIS00/0997 from the Fondo de Investigaciones Sanitarias (Spain). JSC has a grant associated to project PETRI no. PTR1995-0430-OP
PY - 2003/8/1
Y1 - 2003/8/1
N2 - The presence of plasmid-encoded trimethoprim resistant dfr genes is the most common mechanism responsible for the acquisition of trimethoprim resistance. The usual method to detect the presence of these genes is hybridization with specific probes. We describe an alternative, faster and easier method, based on PCR amplification and RFLP analysis, to discriminate up to sixteen different dfr genes.
AB - The presence of plasmid-encoded trimethoprim resistant dfr genes is the most common mechanism responsible for the acquisition of trimethoprim resistance. The usual method to detect the presence of these genes is hybridization with specific probes. We describe an alternative, faster and easier method, based on PCR amplification and RFLP analysis, to discriminate up to sixteen different dfr genes.
UR - http://www.scopus.com/inward/record.url?scp=0041424806&partnerID=8YFLogxK
U2 - 10.1016/S0732-8893(03)00062-2
DO - 10.1016/S0732-8893(03)00062-2
M3 - Artículo
C2 - 12944023
AN - SCOPUS:0041424806
SN - 0732-8893
VL - 46
SP - 295
EP - 298
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -