Detection of dihydrofolate reductase genes by PCR and RFLP

Margarita M. Navia, Joaquim Ruiz, Javier Sanchez-Cespedes, Jordi Vila

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


The presence of plasmid-encoded trimethoprim resistant dfr genes is the most common mechanism responsible for the acquisition of trimethoprim resistance. The usual method to detect the presence of these genes is hybridization with specific probes. We describe an alternative, faster and easier method, based on PCR amplification and RFLP analysis, to discriminate up to sixteen different dfr genes.

Original languageEnglish
Pages (from-to)295-298
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Issue number4
StatePublished - 1 Aug 2003


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