TY - JOUR
T1 - Decreased permeability and enhanced proton-dependent active efflux in the development of resistance to quinolones in Morganella morganii
AU - Del Mar Tavío, María
AU - Vila, Jordi
AU - Ruiz, Joaquím
AU - Manuel Martín Sánchez, Antonio
AU - Teresa Jiménez De Anta, María
PY - 2000/3
Y1 - 2000/3
N2 - Five quinolone-resistant strains were developed from a clinical Morganella morganii isolate (M1 strain) which was susceptible to nalidixic acid and fluoroquinolones. All five in vitro selected mutants showed diminished norfloxacin accumulation and two of them also decreased the expression of the AgO in the outer membrane lipopolysaccharide with respect to their parent strain and to the M. morganii NCTC-235 type strain. Likewise, the M1 strain did not express a 37-38 kDa protein and accumulated less norfloxacin than the M. morganii NCTC-235 strain. The decreased norfloxacin uptake in the five mutants compared with the M. morganii NCTC-235 strain was due to an enhanced proton-dependent active efflux plus a pre-existent decreased expression of a 37-38 kDa protein in the parent strain. Copyright (C) 2000 Elsevier Science B.V.
AB - Five quinolone-resistant strains were developed from a clinical Morganella morganii isolate (M1 strain) which was susceptible to nalidixic acid and fluoroquinolones. All five in vitro selected mutants showed diminished norfloxacin accumulation and two of them also decreased the expression of the AgO in the outer membrane lipopolysaccharide with respect to their parent strain and to the M. morganii NCTC-235 type strain. Likewise, the M1 strain did not express a 37-38 kDa protein and accumulated less norfloxacin than the M. morganii NCTC-235 strain. The decreased norfloxacin uptake in the five mutants compared with the M. morganii NCTC-235 strain was due to an enhanced proton-dependent active efflux plus a pre-existent decreased expression of a 37-38 kDa protein in the parent strain. Copyright (C) 2000 Elsevier Science B.V.
KW - Active-efflux
KW - Morganella morganii
KW - Permeability
KW - Quinolones
KW - Resistance
UR - http://www.scopus.com/inward/record.url?scp=0034099126&partnerID=8YFLogxK
U2 - 10.1016/S0924-8579(00)00117-5
DO - 10.1016/S0924-8579(00)00117-5
M3 - Artículo
C2 - 10720807
AN - SCOPUS:0034099126
VL - 14
SP - 157
EP - 160
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
SN - 0924-8579
IS - 2
ER -