Decreased permeability and enhanced proton-dependent active efflux in the development of resistance to quinolones in Morganella morganii

María Del Mar Tavío, Jordi Vila, Joaquím Ruiz, Antonio Manuel Martín Sánchez, María Teresa Jiménez De Anta

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13 Scopus citations

Abstract

Five quinolone-resistant strains were developed from a clinical Morganella morganii isolate (M1 strain) which was susceptible to nalidixic acid and fluoroquinolones. All five in vitro selected mutants showed diminished norfloxacin accumulation and two of them also decreased the expression of the AgO in the outer membrane lipopolysaccharide with respect to their parent strain and to the M. morganii NCTC-235 type strain. Likewise, the M1 strain did not express a 37-38 kDa protein and accumulated less norfloxacin than the M. morganii NCTC-235 strain. The decreased norfloxacin uptake in the five mutants compared with the M. morganii NCTC-235 strain was due to an enhanced proton-dependent active efflux plus a pre-existent decreased expression of a 37-38 kDa protein in the parent strain. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalInternational Journal of Antimicrobial Agents
Volume14
Issue number2
DOIs
StatePublished - Mar 2000

Keywords

  • Active-efflux
  • Morganella morganii
  • Permeability
  • Quinolones
  • Resistance

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