Cryopreservation effect on sperm viability, mitochondrial membrane potential, acrosome integrity and sperm capacitation of alpaca spermatozoa detected by imaging flow cytometry

Alexei Santiani; Javier Juárez; Pablo Allauca; Brian Roman; Alejandra Ugarelli; Shirley Evangelista-Vargas

doi:10.1111/rda.14318

Cryopreservation effect on sperm viability, mitochondrial membrane potential, acrosome integrity and sperm capacitation of alpaca spermatozoa detected by imaging flow cytometry

Alexei Santiani, Javier Juárez, Pablo Allauca, Brian Roman, Alejandra Ugarelli, Shirley Evangelista-Vargas

Medicina veterinaria y zootecnia

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Eighty-five sperm samples were cryopreserved and SYBR14/PI, MitoTracker Deep Red FM, FITC-PSA/PI and chlortetracycline were used for imaging flow cytometry evaluation of sperm viability, mitochondrial membrane potential (MMP), acrosome integrity and sperm capacitation, respectively. Sperm motility was also registered. Sperm motility (46.1 ± 7.7 vs. 24.1% ± 6.5%), sperm viability (49.8 ± 11.5 vs. 32.3% ± 9.6%) and high MMP (49.8% ± 12.4% vs. 34.9% ± 9.9%) decreased significantly (p <.05) during cryopreservation process, in contrast to acrosome-reacted in viable spermatozoa (1.0% ± 1.6% vs. 1.0% ± 1.0%) and sperm capacitation (10.0 ± 9.8 vs. 8.2% ± 12.4%) that were similar (p >.05) before and after cryopreservation. Positive correlations were found between sperm motility versus high MMP (r =.63), sperm motility versus sperm viability (r =.67) and sperm viability versus high MMP (r =.88). In conclusion, cryopreservation of alpaca spermatozoa is related to a decrease in sperm motility, sperm viability and high MMP, meanwhile acrosome integrity and sperm capacitation are not affected.

Original language	English
Pages (from-to)	560-563
Number of pages	4
Journal	Reproduction in Domestic Animals
Volume	58
Issue number	4
DOIs	https://doi.org/10.1111/rda.14318
State	Published - Apr 2023

Keywords

Vicugna pacos
alpaca
cryopreservation
flow cytometry
spermatozoa

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10.1111/rda.14318

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@article{460fb09fca1a48ef8e956dd6f8e0fcd0,

title = "Cryopreservation effect on sperm viability, mitochondrial membrane potential, acrosome integrity and sperm capacitation of alpaca spermatozoa detected by imaging flow cytometry",

abstract = "Eighty-five sperm samples were cryopreserved and SYBR14/PI, MitoTracker Deep Red FM, FITC-PSA/PI and chlortetracycline were used for imaging flow cytometry evaluation of sperm viability, mitochondrial membrane potential (MMP), acrosome integrity and sperm capacitation, respectively. Sperm motility was also registered. Sperm motility (46.1 ± 7.7 vs. 24.1% ± 6.5%), sperm viability (49.8 ± 11.5 vs. 32.3% ± 9.6%) and high MMP (49.8% ± 12.4% vs. 34.9% ± 9.9%) decreased significantly (p <.05) during cryopreservation process, in contrast to acrosome-reacted in viable spermatozoa (1.0% ± 1.6% vs. 1.0% ± 1.0%) and sperm capacitation (10.0 ± 9.8 vs. 8.2% ± 12.4%) that were similar (p >.05) before and after cryopreservation. Positive correlations were found between sperm motility versus high MMP (r =.63), sperm motility versus sperm viability (r =.67) and sperm viability versus high MMP (r =.88). In conclusion, cryopreservation of alpaca spermatozoa is related to a decrease in sperm motility, sperm viability and high MMP, meanwhile acrosome integrity and sperm capacitation are not affected.",

keywords = "Vicugna pacos, alpaca, cryopreservation, flow cytometry, spermatozoa",

author = "Alexei Santiani and Javier Ju{\'a}rez and Pablo Allauca and Brian Roman and Alejandra Ugarelli and Shirley Evangelista-Vargas",

note = "Publisher Copyright: {\textcopyright} 2023 Wiley-VCH GmbH.",

year = "2023",

month = apr,

doi = "10.1111/rda.14318",

language = "Ingl{\'e}s",

volume = "58",

pages = "560--563",

journal = "Reproduction in Domestic Animals",

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Cryopreservation effect on sperm viability, mitochondrial membrane potential, acrosome integrity and sperm capacitation of alpaca spermatozoa detected by imaging flow cytometry. / Santiani, Alexei; Juárez, Javier; Allauca, Pablo et al.
In: Reproduction in Domestic Animals, Vol. 58, No. 4, 04.2023, p. 560-563.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cryopreservation effect on sperm viability, mitochondrial membrane potential, acrosome integrity and sperm capacitation of alpaca spermatozoa detected by imaging flow cytometry

AU - Santiani, Alexei

AU - Juárez, Javier

AU - Allauca, Pablo

AU - Roman, Brian

AU - Ugarelli, Alejandra

AU - Evangelista-Vargas, Shirley

PY - 2023/4

Y1 - 2023/4

N2 - Eighty-five sperm samples were cryopreserved and SYBR14/PI, MitoTracker Deep Red FM, FITC-PSA/PI and chlortetracycline were used for imaging flow cytometry evaluation of sperm viability, mitochondrial membrane potential (MMP), acrosome integrity and sperm capacitation, respectively. Sperm motility was also registered. Sperm motility (46.1 ± 7.7 vs. 24.1% ± 6.5%), sperm viability (49.8 ± 11.5 vs. 32.3% ± 9.6%) and high MMP (49.8% ± 12.4% vs. 34.9% ± 9.9%) decreased significantly (p <.05) during cryopreservation process, in contrast to acrosome-reacted in viable spermatozoa (1.0% ± 1.6% vs. 1.0% ± 1.0%) and sperm capacitation (10.0 ± 9.8 vs. 8.2% ± 12.4%) that were similar (p >.05) before and after cryopreservation. Positive correlations were found between sperm motility versus high MMP (r =.63), sperm motility versus sperm viability (r =.67) and sperm viability versus high MMP (r =.88). In conclusion, cryopreservation of alpaca spermatozoa is related to a decrease in sperm motility, sperm viability and high MMP, meanwhile acrosome integrity and sperm capacitation are not affected.

AB - Eighty-five sperm samples were cryopreserved and SYBR14/PI, MitoTracker Deep Red FM, FITC-PSA/PI and chlortetracycline were used for imaging flow cytometry evaluation of sperm viability, mitochondrial membrane potential (MMP), acrosome integrity and sperm capacitation, respectively. Sperm motility was also registered. Sperm motility (46.1 ± 7.7 vs. 24.1% ± 6.5%), sperm viability (49.8 ± 11.5 vs. 32.3% ± 9.6%) and high MMP (49.8% ± 12.4% vs. 34.9% ± 9.9%) decreased significantly (p <.05) during cryopreservation process, in contrast to acrosome-reacted in viable spermatozoa (1.0% ± 1.6% vs. 1.0% ± 1.0%) and sperm capacitation (10.0 ± 9.8 vs. 8.2% ± 12.4%) that were similar (p >.05) before and after cryopreservation. Positive correlations were found between sperm motility versus high MMP (r =.63), sperm motility versus sperm viability (r =.67) and sperm viability versus high MMP (r =.88). In conclusion, cryopreservation of alpaca spermatozoa is related to a decrease in sperm motility, sperm viability and high MMP, meanwhile acrosome integrity and sperm capacitation are not affected.

KW - Vicugna pacos

KW - alpaca

KW - cryopreservation

KW - flow cytometry

KW - spermatozoa

UR - http://www.scopus.com/inward/record.url?scp=85147115151&partnerID=8YFLogxK

U2 - 10.1111/rda.14318

DO - 10.1111/rda.14318

M3 - Artículo

AN - SCOPUS:85147115151

SN - 0936-6768

VL - 58

SP - 560

EP - 563

JO - Reproduction in Domestic Animals

JF - Reproduction in Domestic Animals

IS - 4

ER -

Cryopreservation effect on sperm viability, mitochondrial membrane potential, acrosome integrity and sperm capacitation of alpaca spermatozoa detected by imaging flow cytometry

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