Concurrent Detection of Circulating Tumor Cells and Circulating Tumor DNA in Triple-negative Breast Cancer

Henry L. Gómez; Carlos A. Castaneda; Miluska Castillo; James Reuben; Hui Gao; Nancy Suarez; Luis A. Bernabe; Jose M. Cotrina; Jorge Dunstan; Marco Velarde; Julio Abugattas; Henry Guerra

doi:10.31557/apjcc.2021.6.4.373-377

Concurrent Detection of Circulating Tumor Cells and Circulating Tumor DNA in Triple-negative Breast Cancer

Henry L. Gómez, Carlos A. Castaneda, Miluska Castillo, James Reuben, Hui Gao, Nancy Suarez, Luis A. Bernabe, Jose M. Cotrina, Jorge Dunstan, Marco Velarde, Julio Abugattas, Henry Guerra

Medicina humana

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: Circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) provide tumor information in breast cancer. Our objective was to characterize CTCs, and contrasted them with ctDNA PIK3CA mutation in 24 triple-negative breast cancer (TNBC). Methods: CTCs genes were characterized by AdnaTest protocol and ctDNA by digital PCR. Results: We found CTCs genes in 37.5% and ctDNA PIK3CA mutations in 29.16%. Three cases with CTCs genes had concurrent ctDNA PIK3CA mutations. MUC1 or GA733-2 were found in 4 cases, and 3 of them had concurrent ctDNA PIK3CA. CTCs ALDH1/TWIST1 were found in 2 cases, AKT2 in one and PI3Kα in another, and none had concurrent ctDNA PIK3CA mutations. There was no correlation between CTCs and ctDNA detection. All 3 cases with CTC & cDNA concurrent finding underwent death during follow-up. Conclusion: Infrequent concurrent detection of CTC and ctDNA presence suggests that both represent independent processes in TNBC patients, and could identify worst prognosis cases.

Original language	English
Pages (from-to)	373-377
Number of pages	5
Journal	Asian Pacific Journal of Cancer Care
Volume	6
Issue number	4
DOIs	https://doi.org/10.31557/apjcc.2021.6.4.373-377
State	Published - 2021

Keywords

ALDH1
Negative Breast Neoplasms
PIK3CA
Triple
circulating tumor DNA
circulating tumor cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.31557/apjcc.2021.6.4.373-377

Cite this

Gómez, H. L., Castaneda, C. A., Castillo, M., Reuben, J., Gao, H., Suarez, N., Bernabe, L. A., Cotrina, J. M., Dunstan, J., Velarde, M., Abugattas, J., & Guerra, H. (2021). Concurrent Detection of Circulating Tumor Cells and Circulating Tumor DNA in Triple-negative Breast Cancer. Asian Pacific Journal of Cancer Care, 6(4), 373-377. https://doi.org/10.31557/apjcc.2021.6.4.373-377

@article{5589159dd92c450a81ac6e9c70cee10b,

title = "Concurrent Detection of Circulating Tumor Cells and Circulating Tumor DNA in Triple-negative Breast Cancer",

abstract = "Objective: Circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) provide tumor information in breast cancer. Our objective was to characterize CTCs, and contrasted them with ctDNA PIK3CA mutation in 24 triple-negative breast cancer (TNBC). Methods: CTCs genes were characterized by AdnaTest protocol and ctDNA by digital PCR. Results: We found CTCs genes in 37.5% and ctDNA PIK3CA mutations in 29.16%. Three cases with CTCs genes had concurrent ctDNA PIK3CA mutations. MUC1 or GA733-2 were found in 4 cases, and 3 of them had concurrent ctDNA PIK3CA. CTCs ALDH1/TWIST1 were found in 2 cases, AKT2 in one and PI3Kα in another, and none had concurrent ctDNA PIK3CA mutations. There was no correlation between CTCs and ctDNA detection. All 3 cases with CTC & cDNA concurrent finding underwent death during follow-up. Conclusion: Infrequent concurrent detection of CTC and ctDNA presence suggests that both represent independent processes in TNBC patients, and could identify worst prognosis cases.",

keywords = "ALDH1, Negative Breast Neoplasms, PIK3CA, Triple, circulating tumor DNA, circulating tumor cells",

author = "G{\'o}mez, {Henry L.} and Castaneda, {Carlos A.} and Miluska Castillo and James Reuben and Hui Gao and Nancy Suarez and Bernabe, {Luis A.} and Cotrina, {Jose M.} and Jorge Dunstan and Marco Velarde and Julio Abugattas and Henry Guerra",

year = "2021",

doi = "10.31557/apjcc.2021.6.4.373-377",

language = "Ingl{\'e}s",

volume = "6",

pages = "373--377",

journal = "Asian Pacific Journal of Cancer Care",

issn = "2588-3682",

publisher = "West Asia Organization for Cancer Prevention",

number = "4",

}

Gómez, HL, Castaneda, CA, Castillo, M, Reuben, J, Gao, H, Suarez, N, Bernabe, LA, Cotrina, JM, Dunstan, J, Velarde, M, Abugattas, J & Guerra, H 2021, 'Concurrent Detection of Circulating Tumor Cells and Circulating Tumor DNA in Triple-negative Breast Cancer', Asian Pacific Journal of Cancer Care, vol. 6, no. 4, pp. 373-377. https://doi.org/10.31557/apjcc.2021.6.4.373-377

TY - JOUR

T1 - Concurrent Detection of Circulating Tumor Cells and Circulating Tumor DNA in Triple-negative Breast Cancer

AU - Gómez, Henry L.

AU - Castaneda, Carlos A.

AU - Castillo, Miluska

AU - Reuben, James

AU - Gao, Hui

AU - Suarez, Nancy

AU - Bernabe, Luis A.

AU - Cotrina, Jose M.

AU - Dunstan, Jorge

AU - Velarde, Marco

AU - Abugattas, Julio

AU - Guerra, Henry

PY - 2021

Y1 - 2021

N2 - Objective: Circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) provide tumor information in breast cancer. Our objective was to characterize CTCs, and contrasted them with ctDNA PIK3CA mutation in 24 triple-negative breast cancer (TNBC). Methods: CTCs genes were characterized by AdnaTest protocol and ctDNA by digital PCR. Results: We found CTCs genes in 37.5% and ctDNA PIK3CA mutations in 29.16%. Three cases with CTCs genes had concurrent ctDNA PIK3CA mutations. MUC1 or GA733-2 were found in 4 cases, and 3 of them had concurrent ctDNA PIK3CA. CTCs ALDH1/TWIST1 were found in 2 cases, AKT2 in one and PI3Kα in another, and none had concurrent ctDNA PIK3CA mutations. There was no correlation between CTCs and ctDNA detection. All 3 cases with CTC & cDNA concurrent finding underwent death during follow-up. Conclusion: Infrequent concurrent detection of CTC and ctDNA presence suggests that both represent independent processes in TNBC patients, and could identify worst prognosis cases.

AB - Objective: Circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) provide tumor information in breast cancer. Our objective was to characterize CTCs, and contrasted them with ctDNA PIK3CA mutation in 24 triple-negative breast cancer (TNBC). Methods: CTCs genes were characterized by AdnaTest protocol and ctDNA by digital PCR. Results: We found CTCs genes in 37.5% and ctDNA PIK3CA mutations in 29.16%. Three cases with CTCs genes had concurrent ctDNA PIK3CA mutations. MUC1 or GA733-2 were found in 4 cases, and 3 of them had concurrent ctDNA PIK3CA. CTCs ALDH1/TWIST1 were found in 2 cases, AKT2 in one and PI3Kα in another, and none had concurrent ctDNA PIK3CA mutations. There was no correlation between CTCs and ctDNA detection. All 3 cases with CTC & cDNA concurrent finding underwent death during follow-up. Conclusion: Infrequent concurrent detection of CTC and ctDNA presence suggests that both represent independent processes in TNBC patients, and could identify worst prognosis cases.

KW - ALDH1

KW - Negative Breast Neoplasms

KW - PIK3CA

KW - Triple

KW - circulating tumor DNA

KW - circulating tumor cells

UR - http://www.scopus.com/inward/record.url?scp=85217020588&partnerID=8YFLogxK

U2 - 10.31557/apjcc.2021.6.4.373-377

DO - 10.31557/apjcc.2021.6.4.373-377

M3 - Artículo

AN - SCOPUS:85217020588

SN - 2588-3682

VL - 6

SP - 373

EP - 377

JO - Asian Pacific Journal of Cancer Care

JF - Asian Pacific Journal of Cancer Care

IS - 4

ER -

Concurrent Detection of Circulating Tumor Cells and Circulating Tumor DNA in Triple-negative Breast Cancer

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this