TY - JOUR
T1 - Wound healing in Piaractus mesopotamicus supplemented with chromium carbochelate and Saccharomyces cerevisiae
AU - Bortoluzzi, Neida Lucas
AU - Castro, Marcelo Pardi de
AU - Claudiano, Gustavo da Silva
AU - Yunis-Aguinaga, Jefferson
AU - Cueva-Quiroz, Victor Alexander
AU - Moraes, Julieta Rodini Engrácia de
AU - Moraes, Flávio Ruas de
N1 - Publisher Copyright:
© 2017, Universidade de Sao Paulo. Faculdade de Medicina Veterinaria e Zootecnia. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Wound healing and tissue repair are necessary to ensure survival and health of any organism. The aim of this study was to investigate the impact of supplementation with chromium carbochelate (CC) and Saccharomyces cerevisiae (SC) on wound healing in tropical teleost fish Piaractus mesopotamicus. Thus, fish were distributed into four groups: a) control (without supplementation); b) supplemented with 18 mg/kg of chromium carbochelate; c) supplemented with 0.3% of S. cerevisiae and d) supplemented with an association of both supplements. After 105 days of feeding, full-thickness skin incisions (2.0 x 1.0 x 0.25 cm) were performed removing epidermis and dermis. Macroscopic and histologic observations were carried out at 1, 3, 7, 14, 21, 28, and 35 days after wounding to monitor the healing rate. Opposing fronts advanced gradually and faster each day demonstrating a progressive increase in the healing process over time. The inflammatory process was exacerbated and expansive, with an increase in mucous cells and chromatophores. Although no significant differences were observed between groups on wound retraction and microscopic parameters, fish supplemented with CC and SC showed faster re-epithelialization, greater degree of organization of collagen fibers, and higher neovascularization. We concluded that supplementation with S. cerevisiae and chromium carbochelate improves specific aspects of cutaneous healing process in pacu.
AB - Wound healing and tissue repair are necessary to ensure survival and health of any organism. The aim of this study was to investigate the impact of supplementation with chromium carbochelate (CC) and Saccharomyces cerevisiae (SC) on wound healing in tropical teleost fish Piaractus mesopotamicus. Thus, fish were distributed into four groups: a) control (without supplementation); b) supplemented with 18 mg/kg of chromium carbochelate; c) supplemented with 0.3% of S. cerevisiae and d) supplemented with an association of both supplements. After 105 days of feeding, full-thickness skin incisions (2.0 x 1.0 x 0.25 cm) were performed removing epidermis and dermis. Macroscopic and histologic observations were carried out at 1, 3, 7, 14, 21, 28, and 35 days after wounding to monitor the healing rate. Opposing fronts advanced gradually and faster each day demonstrating a progressive increase in the healing process over time. The inflammatory process was exacerbated and expansive, with an increase in mucous cells and chromatophores. Although no significant differences were observed between groups on wound retraction and microscopic parameters, fish supplemented with CC and SC showed faster re-epithelialization, greater degree of organization of collagen fibers, and higher neovascularization. We concluded that supplementation with S. cerevisiae and chromium carbochelate improves specific aspects of cutaneous healing process in pacu.
KW - Chromium carbochelate
KW - Freshwater fish
KW - Pacu
KW - Saccharomyces cerevisiae
KW - Tissue repair
UR - http://www.scopus.com/inward/record.url?scp=85028733782&partnerID=8YFLogxK
U2 - 10.11606/issn.1678-4456.bjvras.2017.124038
DO - 10.11606/issn.1678-4456.bjvras.2017.124038
M3 - Artículo
AN - SCOPUS:85028733782
SN - 1413-9596
VL - 54
SP - 178
EP - 187
JO - Brazilian Journal of Veterinary Research and Animal Science
JF - Brazilian Journal of Veterinary Research and Animal Science
IS - 2
ER -