Characterization of sparfloxacin-resistant mutants of Staphylococcus aureus obtained in vitro

Joaquim Ruiz, Josep M. Sierra, M. Teresa Jiménez De Anta, Jordi Vila

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A sparfloxacin-susceptible clinical isolate of Staphylococcus aureus was grown in increased concentrations of sparfoxacin. The presence of mutations in gyrA, gyrB, grlA and grlB genes was analyzed. The primary point mutation was located in the gyrA gene (Glu-88 to Lys). Two further mutation steps appeared in the amino acid change Ser-80 to Tyr in GrlA. No mutations occurred in the gyrB or grlB genes. Efflux pumps involved in the increase of resistance were also found to affect norfloxacin and ciprofloxacin. This effect may be related to NorA. An overexpression of NorA, may be associated with the increase of the MIC of norfloxacin from 32 mg/l to >200 mg/l in the final mutant. The MICs levels of sparfloxacin were affected by unknown mechanism.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalInternational Journal of Antimicrobial Agents
Volume18
Issue number2
DOIs
StatePublished - 2001

Keywords

  • Efflux pumps
  • GyrA
  • Quinolones
  • Resistance
  • S. aureus
  • Sparfloxacin

Fingerprint

Dive into the research topics of 'Characterization of sparfloxacin-resistant mutants of Staphylococcus aureus obtained in vitro'. Together they form a unique fingerprint.

Cite this