Joaquim Ruiz, Cláudia Gomes, Maria J. Pons

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations


Bartonella spp. is an arthropod-vectorized bacterial genus responsible for a wide array of vector-borne diseases. Although potentially lethal, Bartonella spp. has remained for years out of scope of clinicians. While challenged by the fastidious nature of Bartonella spp., the development of molecular tools has allowed identifying an increasing number of fully or partially characterized new Bartonella species, contributing to the knowledge of its epidemiology and the further understanding of its pathogenicity. The present chapter summarizes the most relevant approaches to type Bartonella spp.

Original languageEnglish
Title of host publicationMolecular Typing in Bacterial Infections, Volume II, Second Edition
PublisherSpringer International Publishing
Number of pages21
ISBN (Electronic)9783030832179
ISBN (Print)9783030832162
StatePublished - 1 Jan 2022


  • 16S rRNA sequencing
  • 16S–23S rRNA intergenic spacer region amplification
  • Amplified Ribosomal DNA Restriction Analysis (ARDRA)
  • Bartonella bacilliformis
  • Bartonella henselae
  • Bartonella quintana
  • Bartonella spp
  • Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
  • Molecular tools
  • Multilocus sequence typing (MLST)
  • Multilocus variable copy numbers of tandem repeats analysis (MLVA)
  • Multispacer typing (MST)
  • Pulsed-field gel electrophoresis (PFGE)
  • Ribotyping
  • Typing PCR


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