Association between PIK3CA Mutations in Blood and Tumor-Infiltrating Lymphocytes in Peruvian Breast Cancer Patients

Carlos A. Castaneda, Miluska Castillo, Luis A. Bernabe, Nancy Suarez, Atocha Romero, Joselyn Sanchez, Ebert Torres, Javier Enciso, Katherine Tello, Nataly Enciso, Marco Velarde, Miguel De La Cruz, Jorge Dunstan, Jose M. Cotrina, Julio Abugattas, Miguel A. Pinillos, Katia Roque, Hugo Fuentes, Ebert Poquioma, Henry GuerraHenry L. Gomez

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To evaluate the relationship between circulating tumor DNA (ctDNA) presence and tumor features including tumor-infiltrating lymphocyte (TIL) levels in Peruvian breast cancer patients. Materials and Methods: This was a prospective study conducted at the Instituto Nacional de Enfemedades Neoplasicas, Peru. We evaluated level of TIL and PIK3CA mutations in ctDNA. Clinical characteristics, including outcome data, were collected from the patient file. Survival was calculated from the date of blood sample drawn to the event time. Data collected were analyzed using SPSS software version 25. Results: We analyzed plasma samples from 183 breast cancer patients. most cases were of Luminal-B (44.8%) phenotype and stage II (41.5%), and median stromal TIL was 30%. PIK3CA mutation in ctDNA was detected in 35% cases (most with E545K) and was associated with lower TIL level (p=0.04). PIK3CA in ctDNA tended to be associated with advanced stages (p=0.09) in the whole series and with higher recurrence rates (p=0.053) in the non-metastatic setting. Patients with presence of PIK3CA in ctDNA tended to have shorter survival (p=0.083). Conclusion: Presence of PIK3CA mutation in ctDNA was frequently found in our Peruvian breast cancer series, was associated with lower TIL levels and tended to predict poor outcomes.

Original languageEnglish
Pages (from-to)3331-3337
Number of pages7
JournalAsian Pacific Journal of Cancer Prevention
Volume23
Issue number10
DOIs
StatePublished - 2022

Keywords

  • Breast cancer
  • Ctdna
  • Lymphocytes
  • Pik3ca

Fingerprint

Dive into the research topics of 'Association between PIK3CA Mutations in Blood and Tumor-Infiltrating Lymphocytes in Peruvian Breast Cancer Patients'. Together they form a unique fingerprint.

Cite this