An insight of rubisco evolution through a multilevel approach

Vladimir Camel, Gaston Zolla

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

RuBisCO is the most abundant enzyme on earth; it regulates the organic carbon cycle in the biosphere. Studying its structural evolution will help to develop new strategies of genetic improvement in order to increase food production and mitigate CO2 emissions. In the present work, we evaluate how the evolution of sequence and structure among isoforms I, II and III of RuBisCO defines their intrinsic flexibility and residue-residue interactions. To do this, we used a multilevel approach based on phylogenetic inferences, multiple sequence alignment, normal mode analysis, and molecular dynamics. Our results show that the three isoforms exhibit greater fluctuation in the loop between αB and βC, and also present a positive correlation with loop 6, an important region for enzymatic activity because it regulates RuBisCO conformational states. Likewise, an increase in the flexibility of the loop structure between αB and βC, as well as Lys330 (form II) and Lys322 (form III) of loop 6, is important to increase photosynthetic efficiency. Thus, the cross-correlation dynamics analysis showed changes in the direction of movement of the secondary structures in the three isoforms. Finally, key amino acid residues related to the flexibility of the RuBisCO structure were indicated, providing important information for its enzymatic engineering.

Original languageEnglish
Article number1761
JournalBiomolecules
Volume11
Issue number12
DOIs
StatePublished - Dec 2021
Externally publishedYes

Keywords

  • Bio3D
  • Cross-correlation dynamics
  • Structural dynamics
  • Structural flexibility

Fingerprint

Dive into the research topics of 'An insight of rubisco evolution through a multilevel approach'. Together they form a unique fingerprint.

Cite this